AFLP

®

technology is a powerful, reproducible

DNA fingerprinting technique that provides 10 to
100 times more information than other approaches
such as RAPDs or RFLPs. AFLP

®

allows for the

selective amplification of restriction fragments
from a digest of genomic DNA. LI-COR

®

's instru-

ments, IRDye

™

AFLP

®

kits and analysis software

provide a complete solution for AFLP

®

research.

There are five basic steps in the AFLP

®

process:

1)

Restriction digest of genomic DNA.

2)

Ligation of oligonucleotide adapters.

3)

Pre-selective amplification.

4)

Selective amplification.

5)

Electrophoresis of the fragments on a
LI-COR instrument.

In addition to a variety of AFLP

®

reagents, there

are several kits available:

•

The Template Preparation Kit is used in
steps one through three of the AFLP

®

process and prepares 100 templates.

•

Selective Amplification Kits are used for
the fourth step in the AFLP

®

process. These

kits selectively amplify 25 templates with
64 different primer combinations, for a total
of 1600 reactions.

•

Startup Kits include both a Template
Preparation Kit and a Selective
Amplification Kit at an economical price.

Increases Accuracy

•

Quality controlled against control band
patterns.

•

Highly reproducible results.

Saves Time

•

Optimized for LI-COR infrared fluorescence-
based instrumentation.

•

Reliable and requires fewer protocol
adjustments.

•

Can be used on uncharacterized genomes.

•

Kits are ready for use.

Saves Money

•

Eliminates costs associated with radio-
activity.

•

Finish projects faster with less labor.

IRDye AFLP

®

Kits

IRDye AFLP

®

Kits

Description

Features and Benefits

The AFLP

®

Startup Kit, above,

includes both a Template Prepara-
tion Kit, shown open at right, and
a Selective Amplification Kit.

®

Ordering information and a list of
related products on back.

4308 Progressive Ave. • P.O. Box 4000 • Lincoln, Nebraska 68504 USA
North America: 800-645-4267 • International: 402-467-0700 • FAX: [phone]
LI-COR GmbH (Germany, Austria, Switzerland): [phone]
LI-COR UK Ltd.: [phone] • www.licor.com

Product

Catalog #

Template Preparation Kit

. . . . . . .

830-06198

Selective Amplification Kits

IRDye

™

700/800

*

. . . . . . . . . .

830-06197

IRDye

™

700 . . . . . . . . . . . . . . .

830-06226

IRDye

™

800 . . . . . . . . . . . . . . .

830-06196

Startup Kits

IRDye

™

700/800

*

. . . . . . . . . .

830-06195

IRDye

™

700 . . . . . . . . . . . . . . .

830-06258

IRDye

™

800 . . . . . . . . . . . . . . .

830-06194

*

Includes IRDye

™

700 E-AAC, E-AAG, E-ACA, E-ACC primers

and the IRDye

™

800 E-ACG, E-ACT, E-AGC, E-AGG primers

IRDye

™

700 Primers – 500 µl, 1000 reactions

E-AAC

*

. . . . . . . . . . . . . . . . . .

829-06300

E-AAG

*

. . . . . . . . . . . . . . . . . .

829-06301

E-ACA

*

. . . . . . . . . . . . . . . . . .

829-06302

E-ACC

*

. . . . . . . . . . . . . . . . . .

829-06303

E-ACG . . . . . . . . . . . . . . . . . .

829-06304

E-ACT . . . . . . . . . . . . . . . . . . .

829-06305

E-AGC . . . . . . . . . . . . . . . . . .

829-06306

E-AGG . . . . . . . . . . . . . . . . . .

829-06307

IRDye

™

800 Primers – 500 µl, 1000 reactions

E-AAC . . . . . . . . . . . . . . . . . . .

829-06308

E-AAG . . . . . . . . . . . . . . . . . .

829-06309

E-ACA . . . . . . . . . . . . . . . . . . .

829-06310

E-ACC . . . . . . . . . . . . . . . . . . .

829-06311

E-ACG

*

. . . . . . . . . . . . . . . . . .

829-06312

E-ACT

*

. . . . . . . . . . . . . . . . . .

829-06313

E-AGC

*

. . . . . . . . . . . . . . . . . .

829-06314

E-AGG

*

. . . . . . . . . . . . . . . . .

829-06315

Mse Unlabeled Primers – 2 ml, 1000 reactions

M-CAA . . . . . . . . . . . . . . . . . .

829-06316

M-CAC . . . . . . . . . . . . . . . . . .

829-06317

M-CAG . . . . . . . . . . . . . . . . . .

829-06318

M-CAT . . . . . . . . . . . . . . . . . .

829-06319

M-CTA . . . . . . . . . . . . . . . . . .

829-06320

M-CTC . . . . . . . . . . . . . . . . . .

829-06321

M-CTG . . . . . . . . . . . . . . . . . .

829-06322

M-CTT . . . . . . . . . . . . . . . . . .

829-06323

Pre-Amp Primer Mix – 2 ml . . .

829-06193

*

Primer included in the IRDye

™

700/800 Selective

Amplification Kit (830-06197)

LI-COR’s 4300 DNA
Analysis System is
designed to deliver the
data necessary to meet the
demands of your research
projects. The system’s
ruggedness and ease of
use make it perfect for
many venues, from high
level research labs to
undergraduate training.
Complete system solutions
include instrument, software, and reagents for
applications ranging from microsatellites and AFLP

®

analysis to reverse genetics (TILLING

®

) and Long Read

DNA sequencing. Contact LI-COR for more information
on the 4300 System.

Size Standards

A 50-700 bp size standard is designed specifically for
AFLP

®

research. These standards are available in two

volumes for each dye:

Product

Catalog #

IRDye

™

700

100 µl (125 lanes) . . . . . . . . . . . . . .

4200-60

2 ml (2500 lanes) . . . . . . . . . . . .

829-05345

IRDye

™

800

100 µl (125 lanes) . . . . . . . . . . . . . .

4000-45

2 ml (2500 lanes) . . . . . . . . . . . .

829-05346

Software

Several software packages are available, depending on
the needs of the AFLP

®

researcher.

Saga

MX

– A highly automated package for

AFLP

®

analysis.

Personal Edition . . . . . . . . . . . . . .

4200-504

Server Edition . . . . . . . . . . . . . . . .

4200-505

Gene ImagIR

– a flexible package that addresses a

variety of sizing and quantification applications.
Gene ImagIR . . . . . . . . . . . . . . . . .

4000-80

AFLP

®

Quantar

™

Pro

– Specialized software for

co-dominant scoring of LI-COR AFLP

®

data.

Contact Keygene Products for details

(www.keygeneproducts.com).

®

LI-COR, IRDye, Saga and Gene ImagIR are registered trademarks or trademarks of LI-COR, Inc.
AFLP

®

and Quantar are registered trademarks of Keygene N.V

TILLING is a registered trademark of Anawah, Inc.
All other trademarks belong to their respective owners.

Ordering Information

Related Products

Doc #982-07526
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