007 9

ruoeuonat ?«ro»p	Amino Acid Rtsidue	r>K,in Free Amino Acid pKa	in Model Peplides
«-~C0OH	C-terminal	1.8 - 2.6	3.1-3.7
3—COOH	A spanie acid	3.9	4.4 - 4.6
1~~ COOH	Cluiamic acid	4.3	4.4 - 4.6
/t-hh -O u + M	. HtSSidirw	6.0	6.5 - 7.0
a —.»H,^+	N-terminai	8.8 - 10.8	7.6 - 8.0
—SH	Cystę? fi e	8.3	8.5 - 8.8
e—nh3^+	Łysinę	10.8	tO.O - 10.2
-o-	Tyrosine	10.9	9.6 - 10.0
— NH—C —NH j * + NHj	Argininc	J2.5	>n
-OM	Scrinc & Thieonine	> 13
Reagent a-Haloacetyl compounds (e.g. ICH2COOH>		Group specifictty r.— N H ~SH ““‘N, ~S“CH3 ~ * N ’	■^nh*."0"^c

N-Maleitnides (e.g. N-ethylmateimide > Mercuriais (e.g. ciHg-^^COOH ) Disulfides ($.g.    ^{S S    N}°

COOH

^F~C)~

Aryi Hałides (e.g.

HOa

* ) COOH

no₂

Acid anhydndcs (e.g.

o

0

I$ocyanate$ (e.g. HNCO )