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Figurę 3. Effect of VSV G mutants on cellular protein synthesis rates.

L929 cells were infected at an MOI of 20 or mock-infected. Cells were labeled with ³⁵S-methionine/cysteine (50pCi/mL) at 4, 8, and 12 hours post-infection. Lysates were subjected to SDS-PAGE, and labeled proteins were ąuantified. (A) Representative image of an autoradiography film showing radio-labeled proteins at 8 hours post-infection. Positions of viral proteins are indicated on the right. (B) The labeled G proteins in images similar to that of panel A were ąuantified using the Alphalmager 3400 software Results, corrected for the background, are shown as a percentage of the WT-infected control at 4 hours post-infection and are the mean ± SEM of four independent experiments. (C) Acid-precipitable radioactivity was measured by scintillation counting for the determination of total protein synthesis. Results are shown as a percentage of the mock-infected control and are the mean ± SEM of three independent experiments, *P <