869568991

Figurę 10. Schematic representation of the data calculation process with the Infinicyt software based on the 'nearest neighbor' principle. First, one event from a celi population (B-cells highlighted in red) in a is identified in a first data file (tubę 2 of B-cell chronic lymphoproliferative disorders (B-CLPD) panel) based on the backbone markers; then the event corresponding to the nearest neighbor of this event is identified in the second data file (right; tubę 5 of B-CLPD panel) as that event occupying the same (dosest) position in a multidimensional space formed by the same backbone parameters (b). Third, through the data calculation process the values for those parameters that were only measured for the later event in the second data file (d) but not for the former event in the first data file are assigned to the said event in the first data file and vice versa (c). Finally, the calculation process is completed for all other events in the celi population of interest (red events). Through this approach, all events in the merged and calculated data file have information about each of the parameters measured in both tubes (e).

antibody panels. Such a library can be used for (1) further evaluation of the utility and performance of antibody panels and (2) pattern-guided prospective dassification of new cases diag-nosed in different individual laboratories, which use the same EuroFlow antibody panels and laboratory procedures.⁵⁰

Evaluation of the EuroFlow antibody panels based on comparisons of groups of reference data files

The EuroFlow 8-color antibody panels for the diagnosis and dassification of hematological malignancies are designed to answer specific clinical questions, which can be grouped into two generał categories: (1) Is a given hematopoietic celi population normal or reactive/regenerating or abnormal/neoplas-tic? (2) When an abnormal/neoplastic celi population is identified, which WHO disease category does it belong to? In order to evaluate the utility and performance of the EuroFlow antibody panels, different groups of reference files that had been stained with the same antibody panels have been constructed. To answer the first question, reference data files from a normal/reactive celi population were compared with their neoplastic counterpart from

2012

>-2010