After being filtered through glass wool, the biofuel collected from the
chemoreactor was injected into Gas chromatography coupled to time-of-flight mass spectrometry GC-TOF MS (LECO Pegasus III) using the splitless mode. The analyses were carried out with an autosampler (CTC Analytics) integrated to the GC system (Agilent Technologies, 6890N) fitted with a 30m X 0.25 mm i.d. capillary column (0.25μιη film of 5% diphenyl-95 dimethyl polysiloxane). Helium gas was used as a carrier gas, and the temperature was ramped from 50 to 300°C at 15°C min"1 following injection. The select mass ion m/z 74 was used to quantify the amount of FAME in the samples based on both internal and external standards added