■CCTGAGGAG
•GGACTCCTC
I
CCTGAGGAG
GGACTCCTC
_ _CCTGTGGA&
_ GGACACCTC
PCR amplify 100- 200 bp
fragment encompassing the
site of the mutation y
i CCTGTGGAG
millions of copies GGACACCTC
separate the strands of the PCR Products by polyacrylamide gel electrophoresis
ł