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Chronuitographic Fingerprints ofTwenty Salvia Species 519

Table /. The sagę (Saluia L.) species irwestigated

No.	Name
1	S. azurea
2	S. \avandulifo\ia
3	S. verticillata
4	S. pratensis
5	S. słaminea
6	S. deserta
7	S. cadmica
8	S. fbrskaohlei
9	S. sclarea
10	S. canariertsis
11	S. hiarts
12	S. triloba
13	S. glutinosa
14	S. nemorosa
15	S. t£squicoIa
16	S. amplexicaulis
17	S. atropalana
18	S. stepposa
19	S. jurisicii
20	S. officinalis

Accelerated Solvent Extraction (ASE)

Compounds of interest (flavonoids and phenołic acids) were extracted from the plant materiał by use of Dionex (Surtnyvale, CA, USA) ASE 200 equip-ment. Each sagę species (5 g) was weighed, powdered in a porcelain mortar, and placed in the celi of the accelerated solvent extraction (ASE) unit. We used a two-step extraction procedurę. In the first step, sagę samples were extracted for 20 min with n-hexane at 40°C, to remove chlorophyll from the foliage, and the n-hexane extract was discarded. In the second step, the same sagę samples were extracted for 12 min with methanol at 100°C. Both extraction steps were performed at a pressure of 65 atm.

Each extract was evaporated to dryness in a stream of air, then 5 mL methanol was added to the dry residue and the mixture was uitrasonicated for 15 min in a model RK 255H Sonorex Super ultrasonication bath (Bande-lin, Berlin, Germany). Finally, the contents of each vial were concentrated to 1 mL by evaporation of excess methanol. Extracts were filtered through an Anotop syringe filter with aluminium oxide adsorbent (Merck #11320) and the samples were ready for liquid chromatographic analysis. The amount analysed was 40 [iL.