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COLUMN LOADING CAPACITY

k    0

Sample Weight Injected

Retention time and peak width are independent of the amount of sample injected up to a point called the column capacity (0). Above this point, retention times(k) decrease and peak widths increase. When retention decreases by 10% of its normal value, the column capacity has been exceeded. Increases in peak width can cause overlap with adjacent peaks, reducing the purity of collected fractions. Analytical scalę columns have capacities on the order of 1mg, while preparative scalę columns can separate tens of milligrams or even grams depending on the diameter of the column.

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