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In vivo studies

Alł procedures were approved by the INRS Institutionai Animal Care and Use Committee. To establish subcutaneous tumors, 5 ^x 10⁵ B16 or B16gp33 cells in 100 pi PBS were injected into the flank of C57B1/6 mice. Six days after tumor implantation, mice were injected with cobra venom factor (CVF; 20 pg/mouse; Quidel, Santa Clara, CA) intraperitoneally. For NK depletion experiments, 200 pg of purified monoclonal anti-NKl.l antibodies ffom PK136 hybridoma, kindly provided by Dr. Suzanne Lemieux (Laval, Canada), were injected intraperitoneally two days pnor to decomplementation (Fig. S2).

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