869568996

channel of false-positive events (data not shown), in linę with previous obsen/ations.²⁴ Morę recently, such instability has also been related to a cell-dependent degradation phenomenon.³⁰ In addition, APCCy7 showed great lot-to-lot differences in brightness and compensation needs (data not shown). AF700 showed little spillover into this latter channel (Table 2), but this dye required the use of a different mirror and filter -680 nm long pass (LP) and 710/ 50 nm band pass (BP), respectively- than those available by default in all four flow cytometers evaluated. In addition, the fluorescence intensity of AF700 translated into suboptimal discrimination of some antigens expressed at relatively Iow levels, particularly when they were expressed on cells that had a bright APC signal (decreased SI due to compensation-induced data spread; data not shown). Finally, the APCH7 dye, a morę stable APC-based tandem dye with a long Stoke's shift, was tested. It showed a lower SI and MFI than its equivalent APCCy7-antibody