• Lecture:
Particular lectures contents |
Number of hours |
1 Introduction. Chemical bonds In biochemistry. Entropy and the laws of thermodynamics. Protein structure and function: amino acids and primary structure |
3 |
2. Protein structure and function - cont. - secondary, tertiary and quaternary structure, Anfinsen experiment, protein folding |
2 |
3.Exploring proteins - the purification of proteins: gel filtration, affinity chromatography, ion-exchange chromatography, ultracentrifugation, activity tests, gel electophoresis, evaluation of purification scheme: quantification of a purification protocol (yield, level), amino acid sequencing - Edman degradation, mass spectrometry |
3 |
4. Exploring proteins - cont. - immunology provides important techniques with which to investigate proteins, automatic solid-phase peptide synthesis, three-dimensional protein structure: NMR spectroscopy and X-ray crystallography, proteom description |
3 |
5.Hemoglobin - portrait of allosteric protein - allosteric regulators with 2,3-BPG, hydrogen ions and C02, hemoglobin transports oxygen efficiently by binding oxygen cooperatively, the Bohr effect, sicie anemia |
2 |
6.Enzymes - basie concepts and kinetics - cofactors for activity, and the reaction spontaneity, the active sites of enzymes, the formation of the transition State ES, the Michaelis-Menten model, the significance of Km and Vmav, the kca,/Km criterion, enzyme specific inhibitors, competitive and noncompetitive inhibition, catalytic antibodies, penicillin, vitamines |
3 |
7.Catalytic strategies - proteases: facilitating a difficult reaction, carbonic anhydrases, restriction enzymes, nucleoside kinases |
2 |
8.Regulatory strategies - aspartate transcarbamoylase, isoenzymes covalent modification, specific proteolytic cleavage, blood clotting |
2 |
9. Carbohydrates - complex carbohydrates conformation, glycoproteins, lectins |
1 |
10.Lipids and celi membranes - common features, constituents of lipids, bilayer permeability, the fluid model mosaic model, compartments in eucaryotic cells |
3 |
11.Membranę channels and pumps - the transport of molecules across a membranę may be active or passive, ATPases protein family, multidrug resistance, secondary transporters, patch-clamp conductance measurements, specific voltage-gated channels, gap junctions |
3 |
12.Metabolism - basie concepts and design - coupled reactions, regulatory strategies, evolution of metabolic pathways |
3 |
• Classes - the contents:
19