Sir Alec Jeffreys
1
DNA profiling
Use of DNA
DNA typing
DNA fingerprinting
Discovered by Professor
Sir Alec Jeffreys
2
Restriction Fragment Length Polymorphism (RFLP)
Why?
Use of DNA
Needed large amounts of sample
Use non-coding region of DNA
3
Use of DNA
Most of what makes us human
beings is common to us all
Few minor differences
4
Coding region
Not highly individualised
Use of DNA
Junk DNA
High variability
5
Can use more than one DNA probe
RFLP
Bill Clinton
7 probes used
Higher probability
More probes
6
Analogous to TLC
Gel Electrophoresis
Capillary gel electrophoresis
Analogous to HPLC
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