1254394716

1254394716



129

exposure protocol (Swanson et al.y 1996). This procedurę consisted in decreasing ambient temperaturę by 3°C every 20 minutes, with trials starting at 0°C. Trials ended when birds became hypothermic, which was detectable in real time as a steady decline in oxygen consumption for several minutes, time at which we measured the bird’s Mb and Tb again. We assumed a bird had reached its Msum when Tb after a trial was < 38°C (Cooper & Gessaman, 2005) (mean Tb after Msum = 33.4 ± 0.3°C). Data from birds with Tb above this threshold were discarded. Mb measured before and after trials were averaged and these values were used for Msum analyses. Birds were brought back to their cage with food and water ad libitum until BMR measurement.

We measured the BMR of the four birds simultaneously from 19:00 to 06:00. Measurements were done at 30°C (within the thermoneutral zonę for this species, Chaplin, 1976) using a constant flow of C02-free dry air (average 470 ml.min'). As for Msum, birds were weighed before and after measurements and average Mb were used in BMR analyses.

Oxygen analyzers were adjusted each day to 20.95% O2 using C02-free dry air. Mass flow valves (Sierra Instruments, Side-Trak® Model 840, Monterey, CA, USA) were calibrated for air and helox using a bubble-O-meter (Dublin, OH, USA) once per winter. Metabolic ratę calculations were done with ExpeData software, v 1.2.6 (Sabie Systems, Las Vegas, NV, USA). Using a 20 sec sampling interval for BMR and a 5 sec interval for Msum, BMR and Msum calculations were based respectively on the lowest and highest averaged 10 minutes of oxygen consumption per measurement sequence according to Lighton’s equation 10.1 (Lighton, 2008). We applied the instantaneous measurement technique (Bartholomew et al.1981) for Msum calculations and a steady State approach for BMR. Duration of BMR trials (around 11 hours) insured that birds were post-absorptive at time of BMR measurement. Since birds use lipids as fuel during fasting and for shivering (Swanson, 2010), we estimated energy consumption using a constant equivalent of 19.8 kJ.L'1 O2 and converted to watts (Gessaman & Nagy, 1988). After BMR measurements, birds were put back in their cage with access to food and water until release on their capture site around 2 hours later. In total, 180 Msum and 221 BMR were recorded for 101 and 114 individuals, respectively.



Wyszukiwarka

Podobne podstrony:
129 exposure protocol (Swanson et al.y 1996). This procedurę consisted in decreasing ambient tempera
Co to jest różnorodność biologiczna (bioróżnorodność)? Begon et al. 1996: Bogactwo gatunkowe - liczb
12 M. Sajewicz et al. From this comparison it is apparent that for three of the five sagę species in
Plant virology in sub-Saharan Africa 62% have been reported (Estelitta et al. 1996). The virus is cu
134 macrophages (Hardwick et al., 1996) ont demontre que Fexposition aux LDLox les rend susceptibles
following infection while the Mmsir mutant has lost this ability (Janelle et al., 2013). This endopl
280 Z. KORUBA ET AL. six degrees of freedom in the discrete model of the system described by ana-lyt
16 H. Pridalova et al. value of fat content in milk was determined to be at 34.70 g.l 1. Growing fat
Zdjęcie290 KIRUNA - magmowe zło2e Fe-P Harlov et al. (2002) - Apatite-monazite relatlon* In the Kllr
P.-J. Royer et al. 408 Cul turę Medium and DC Maturation uncicr GMP conditions, thc culnirc protocol
148 B. Rihn et aUToxicologv 109 (1996) 147-156 undcrstood (Walker et al., 1992; Brody, 1993) while s
152 B. Rihn et al. I To.ricology 109 (1996) 147-156 Table 2 Cellular and TNF-st response to crocidol
154 B. Rihn et al. IToxicology 109 (1996) 147-156 with, however very large individual variations. Al
155 B. Rihn et aL / Toxicology 109 (1996) 147-156 toe, 1983) using relatively high dust concentra-io
120 T. Wilgat et al. significance, as well as an upper part of Cretaceous rocks. This zonę does not
128 T. Wilgat et al. Polish Kingdom. This lakę was so shallow that after digging a ditch it ran off,
30 to winter constraints (Vezina et al., 2011), within individual data to support this statement are

więcej podobnych podstron