Kultury komórek
Kultury komórek
Zagadnienie dotyczy:
Bakterii
Grzybów
Wirusów
Hodowli komórek zwierzęcych i roślinnych
Kto może tworzyć ?
Kolekcje tworzą jednostki naukowe
oraz gospodarcze
The DSMZ microbial collections contain over 15 000 cultures
representing some 6 900 species and 1 400 genera (
,
,
,
).
Deutsche Sammlung von Mikroorganismen
und Zellkulturen GmbH German Collection
of Microorganisms and Cell Cultures
The European Collection of Cell Cultures (ECACC)
was established in 1984. It is recognised as an
international Depository Authority under the
Budapest Treaty. The cell lines available include
those stored by the ECACC, the European
Human Cell Bank and the Hybridoma Collection;
it is also able to supply cell lines held by ATCC.
http://www.biotech.ist.unige.it/cldb/cat5.html
Lactobacillus acidophilus (Moro) Hansen and Mocquot BT1806
Lactobacillus acidophilus (Moro) Hansen and Mocquot BT
1389
Lactobacillus acidophilus (Moro) Hansen and Mocquot
4B [M. Rogosa 205X, NCDO 1, NCIB 1723, NCTC 1723]
Lactobacillus acidophilus (Moro) Hansen and Mocquot deposited as
Bacillus acidophilus Moro [43]
Lactobacillus acidophilus (Moro)
Hansen and Mocquot deposited as Lactobacillus bulgaricus (Orla-
Jensen) Rogosa and Jensen [VPI 11091]
About the ATCC-LGC Standards Partnership
LGC Standards' partnership with ATCC facilitates the distribution of
ATCC cultures and bioproducts to life science researchers
throughout Europe and India. Our specific aims are to make access
to the important resources of ATCC are more easily accessible to
the European scientific community through a local stock holding of
more than 5000 individual culture items supported by our local
office network delivering the highest levels of customer service
and technical support.
www.lgcstandards-atcc.org/
Zasady tworzenia
Dokładny opis pochodzenia
Dokładna identyfikacja biochemiczna
Identyfikacja genetyczna
Wykaz cech przydatnych dla odbiorcy
Zasady przechowywania:
Zamrożenie – 80 st C, - 196 st. C ( ciekły
azot)
Zamrożenie - 20 st C w specjalnych
pożywkach (w 10% ) glicerolu
Liofilizacja
Zastosowanie:
Badanie antybiotykooporności
Badanie podłoży bakteriologicznych
Badanie dezynfektantów
Badanie procesów sterylizacyjnych
Badanie patogennosci bakterii i wirusów
Akredytacja laboratoriów ( szczepy referencyjne
The culture medium contains a gel (agar) with the proper mixture of nutrients, sugars,
vitamins and hormones, which causes the plant part to grow at very rapid rates to
produce new plantlets. It has been estimated that one chrysanthemum apex placed in
tissue culture could produce up to 1,000,000 new plantlets in one year. Thus, tissue
culture is used for rapid multiplication of plants. A very specialized laboratory is required
for tissue culture. All the procedures are done in a laboratory and special ventilated
cabinet that is as sterile as an operating room.
David Wm. Reed, Instructor
Department of Horticultural Sciences
Texas A&M University
Human HeLa Cells grown in tissue culture and stained with
(red) and
mouse monoclonal panspecific nuclear pore complex antibody MCA-39C7
(green). The vimentin filaments in the cytoplasm of the cells are clearly visible in red,
while the nuclear pore complex is also visible in green
©EnCor Biotechnology Inc. 2007.
Rat glial cells in mixed culture stained with affinity purified
(red). Hoechst dye reveals nuclear DNA in blue.
©EnCor Biotechnology Inc. 2007.
Glial fibrillary acidic protein (GFAP) is an
(IF)
that is found in
such as
, but also in other cell
types such as
in the testis and
in the liver.
First described in 1971,
GFAP is a type III IF protein that maps, in
humans, to 17q21.
It is closely related to its non-epithelial family
members,
,
, and peripherin, which are all involved in the
structure and function of the cell’s cytoskeleton. GFAP is thought to help
to maintain astrocyte mechanical strength, as well as the shape of cells
but its exact function remains poorly understood, despite the number of
studies using it as a cell marker.
http://en.wikipedia.org/wiki/Glial_fibrillary_acidic_protein
Butelki do kultur komórkowych w zawiesinie
Wykonane z polistyrenu . Sterylne. Z białą nakrętką. Idealne do
kultur komórkowych w zawiesinie dzięki bardzo hydrofobowej
powierzchni. Wysoko osadzona nachylona szyjka butelki chroni...
To generate human ES cell cultures, cells from the inner cell mass
of a human blastocyst were cultured in a multi-step process. The
pluripotent cells of the inner cell mass were separated from the
surrounding trophectoderm by immunosurgery, the antibody-
mediated dissolution of the trophectoderm. The inner cell masses
were plated in culture dishes containing growth medium
supplemented with fetal bovine serum on feeder layers of mouse
embryonic fibroblasts that had been gamma-irradiated to prevent
their replication. After 9 to 15 days, when inner cell masses had
divided and formed clumps of cells, cells from the periphery of
the clumps were chemically or mechanically dissociated and
replated in the same culture conditions. Colonies of apparently
homogeneous cells were selectively removed, mechanically
dissociated, and replated. These were expanded and passaged,
thus creating a cell line. None of the initial 5 human ES cell lines
generated in this manner was derived clonally (cloned from a
single cell and are, therefore, genetically identical