Esteban Ballestar Epigenetic alteration in autoimmune disease


Ballestar et al. Journal of Translational Medicine 2011, 9(Suppl 2):I3
http://www.translational-medicine.com/content/9/S2/I3
INVITED LECTURE PRESENTATION Open Access
Epigenetic alterations in autoimmune disease
Esteban Ballestar1*, Biola M Javierre1, Lorenzo de la Rica1, David Gómez-Cabrero2, Jesper Tegnér2,
Carmen Gomez-Vaquero3, Javier Narvaez3, Henar Hernando1, Virginia C Rodriguez1, Roser Vento1, Laura Ciudad1,
Javier Rodriguez-Ubreva1
From 6th European Workshop on Immune-Mediated Inflammatory Diseases
Nice, France. 23-25 November 2011
Background changes is then explored by using experimental models
In the past years, we have witnessed unprecedented atten- where these factors can be overexpressed or knocked-
tion for the study of epigenetic alterations in the context down. Also, their direct association at specific genomic
of a variety of complex disorders, including autoimmune sites can be investigated by using chromatin immunopreci-
diseases [1]. This is in part due to the observation that pitation assays.
genetics is insufficient to entirely explain the predisposi-
tion to their pathogenesis. The environmental influence is Results
well illustrated by the existence of partial concordance for High-throughput and candidate sequence analysis of
susceptibility to disease in monozygotic twins. In connec- DNA methylation in samples from different autoimmune
tion with this epigenetic mechanisms regulate gene diseases show the existence of alterations associated with
expression and are sensitive to external stimuli, bridging these diseases. We have also identified DNA methylation
the gap between environmental and genetic factors. There changes in association with several factors associated
is now considerable evidence of the existence of epigenetic with autoimmune disease like infection with Epstein
alterations, particularly DNA methylation alterations, in Barr-virus of B cells. Most of the changes observed occur
diseases like systemic lupus erythematosus or rheumatoid in the direction towards hypomethylation associated with
arthritis [1]. Most of the studies were initially performed gene upregulation.
by using candidate-gene approaches, although the increas-
ing availability of high-throughput methods is providing Conclusions
better methods for the screening of epigenetic alterations Our findings are providing potentially relevant DNA
in these diseases [2]. methylation markers for the clinical managements of dif-
ferent autoimmune diseases and also support the notion
Materials and methods that epigenetic alterations may be critical in the pathogen-
We have used different methylation bead arrays to profile esis of autoimmune disease.
the DNA methylation status of CpG sites in both samples
obtainedfrompatients fromdifferent autoimmune diseases
Author details
and normal individuals as well as different experimental 1
Chromatin and Disease Group, Cancer Epigenetics and Biology Programme
models that allow to explore conditions where the immune (PEBC), Bellvitge Biomedical Research Institute (IDIBELL), L Hospitalet de
2
Llobregat, Barcelona, Spain. Dept. of Medicine, Karolinska Institutet,
tolerance is lost. Following bioinformatics analysis, we indi-
Computational Medicine Unit, Centre for Molecular Medicine, and Swedish
vidually validate relevant genes by bisulfite pyrosequencing 3
e-science Research Centre (SeRC), Solna, Stockholm, Sweden. Dept. of
and check their expression levels by quantitative RT-PCR. Rheumatology, Bellvitge University Hospital, L Hospitalet de Llobregat,
Barcelona, Spain.
The potential role of different chromatin factors in these
Published: 23 November 2011
References
1. Ballestar E: Epigenetics Alterations in Autoimmune Rheumatic Diseases.
1
Chromatin and Disease Group, Cancer Epigenetics and Biology Programme
Nature Reviews Rheumatology 7:263-271.
(PEBC), Bellvitge Biomedical Research Institute (IDIBELL), L Hospitalet de
Llobregat, Barcelona, Spain
Full list of author information is available at the end of the article
© 2011 Ballestar et al; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons
Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in
any medium, provided the original work is properly cited.
Ballestar et al. Journal of Translational Medicine 2011, 9(Suppl 2):I3 Page 2 of 2
http://www.translational-medicine.com/content/9/S2/I3
2. Javierre B, et al: Changes in the Pattern of DNA Methylation Associate
with Twin Discordance in Systemic Lupus Erythematosus. Genome Res
2010, 20:170-179.
doi:10.1186/1479-5876-9-S2-I3
Cite this article as: Ballestar et al.: Epigenetic alterations in autoimmune
disease. Journal of Translational Medicine 2011 9(Suppl 2):I3.
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