Agata Pietrucka
Biotechnologia IV
Wybrany gen: Homo sapiens forkhead box P2 (FOXP2) on chromosome 7.
Zadanie1:
Late-postnatal cannabinoid exposure persistently increases FoxP2 expression within zebra finch striatum.
Department of Pharmacology and Toxicology, Brody School of Medicine, East Carolina University, Greenville, North Carolina 27834.
Dev Neurobiol. 2009 Dec 16. [Epub ahead of print]
PMID: 20017118 [PubMed - as supplied by publisher]
Zadanie 2:
Fisher SE, Scharff C.
FOXP2 as a molecular window into speech and language.
Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford, OX3 7BN, UK. simon.fisher@well.ox.ac.uk
Trends Genet. 2009 Apr;25(4):166-77. Epub 2009 Mar 21.
PMID: 19304338 [PubMed - indexed for MEDLINE]
Zadanie 3:
Homo sapiens forkhead box P2 (FOXP2), transcript variant 1, mRNA -NM_014491.2
Sekwencja cDNA:
>gi|149999354|ref|NM_014491.2| Homo sapiens forkhead box P2 (FOXP2), transcript variant 1, mRNA
CTTGAACCTTTGTCACCCCTCACGTTGCACACCAAAGACATACCCTAGTGATTAAATGCTGATTTTGTGT
ACGATTGTCCACGGACGCCAAAACAATCACAGAGCTGCTTGATTTGTTTTAATTACCAGCACAAAATGCC
ATCAGTCTGGGACGTGATCGGGCAGAGGTGTACTCACAGTAGTGTAAATACTGCTGTAAATAGTTGTCTG
ATGGTGGCTTTGACAGTGAGCTAGCTTCTGAGTTTTCCCTTCTTTTTATACTGTTTTCTGTGCTGGCTTT
TTTGAATCTTCCTAATTTTTCATCTCTTTAACAAACTCCTATGAAGTTGAAACCGGGAAGTTTGCTCTAA
CATTTCCAGAGAAGGTATTAAGTCATGATGCAGGAATCTGCGACAGAGACAATAAGCAACAGTTCAATGA
ATCAAAATGGAATGAGCACTCTAAGCAGCCAATTAGATGCTGGCAGCAGAGATGGAAGATCAAGTGGTGA
CACCAGCTCTGAAGTAAGCACAGTAGAACTGCTGCATCTGCAACAACAGCAGGCTCTCCAGGCAGCAAGA
CAACTTCTTTTACAGCAGCAAACAAGTGGATTGAAATCTCCTAAGAGCAGTGATAAACAGAGACCACTGC
AGGTGCCTGTGTCAGTGGCCATGATGACTCCCCAGGTGATCACCCCTCAGCAAATGCAGCAGATCCTTCA
GCAACAAGTCCTGTCTCCTCAGCAGCTACAAGCCCTTCTCCAACAACAGCAGGCTGTCATGCTGCAGCAG
CAACAACTACAAGAGTTTTACAAGAAACAGCAAGAGCAGTTACATCTTCAGCTTTTGCAGCAGCAGCAGC
AACAGCAGCAGCAGCAACAACAGCAGCAACAACAGCAGCAGCAACAACAACAACAACAGCAGCAACAACA
GCAGCAGCAGCAGCAACAGCAGCAGCAGCAGCAACAGCATCCTGGAAAGCAAGCGAAAGAGCAGCAGCAG
CAGCAGCAGCAGCAACAGCAATTGGCAGCCCAGCAGCTTGTCTTCCAGCAGCAGCTTCTCCAGATGCAAC
AACTCCAGCAGCAGCAGCATCTGCTCAGCCTTCAGCGTCAGGGACTCATCTCCATTCCACCTGGCCAGGC
AGCACTTCCTGTCCAATCGCTGCCTCAAGCTGGCTTAAGTCCTGCTGAGATTCAGCAGTTATGGAAAGAA
GTGACTGGAGTTCACAGTATGGAAGACAATGGCATTAAACATGGAGGGCTAGACCTCACTACTAACAATT
CCTCCTCGACTACCTCCTCCAACACTTCCAAAGCATCACCACCAATAACTCATCATTCCATAGTGAATGG
ACAGTCTTCAGTTCTAAGTGCAAGACGAGACAGCTCGTCACATGAGGAGACTGGGGCCTCTCACACTCTC
TATGGCCATGGAGTTTGCAAATGGCCAGGCTGTGAAAGCATTTGTGAAGATTTTGGACAGTTTTTAAAGC
ACCTTAACAATGAACACGCATTGGATGACCGAAGCACTGCTCAGTGTCGAGTGCAAATGCAGGTGGTGCA
ACAGTTAGAAATACAGCTTTCTAAAGAACGCGAACGTCTTCAAGCAATGATGACCCACTTGCACATGCGA
CCCTCAGAGCCCAAACCATCTCCCAAACCTCTAAATCTGGTGTCTAGTGTCACCATGTCGAAGAATATGT
TGGAGACATCCCCACAGAGCTTACCTCAAACCCCTACCACACCAACGGCCCCAGTCACCCCGATTACCCA
GGGACCCTCAGTAATCACCCCAGCCAGTGTGCCCAATGTGGGAGCCATACGAAGGCGACATTCAGACAAA
TACAACATTCCCATGTCATCAGAAATTGCCCCAAACTATGAATTTTATAAAAATGCAGATGTCAGACCTC
CATTTACTTATGCAACTCTCATAAGGCAGGCTATCATGGAGTCATCTGACAGGCAGTTAACACTTAATGA
AATTTACAGCTGGTTTACACGGACATTTGCTTACTTCAGGCGTAATGCAGCAACTTGGAAGAATGCAGTA
CGTCATAATCTTAGCCTGCACAAGTGTTTTGTTCGAGTAGAAAATGTTAAAGGAGCAGTATGGACTGTGG
ATGAAGTAGAATACCAGAAGCGAAGGTCACAAAAGATAACAGGAAGTCCAACCTTAGTAAAAAATATACC
TACCAGTTTAGGCTATGGAGCAGCTCTTAATGCCAGTTTGCAGGCTGCCTTGGCAGAGAGCAGTTTACCT
TTGCTAAGTAATCCTGGACTGATAAATAATGCATCCAGTGGCCTACTGCAGGCCGTCCACGAAGACCTCA
ATGGTTCTCTGGATCACATTGACAGCAATGGAAACAGTAGTCCGGGCTGCTCACCTCAGCCGCACATACA
TTCAATCCACGTCAAGGAAGAGCCAGTGATTGCAGAGGATGAAGACTGCCCAATGTCCTTAGTGACAACA
GCTAATCACAGTCCAGAATTAGAAGACGACAGAGAGATTGAAGAAGAGCCTTTATCTGAAGATCTGGAAT
GAGAACTGACTTGTGAAACCTCAGCGTGAAGGGACATATCACTGACCTTCATAACCACTCCACAACCATG
AATATTTGACAAATTTTTACTGTGACTATTTATTAAGCATGGATAAAGGAGACAGCCCTAAAGGAACTTA
CTAAGCCAGCCCTTTGGGATTCAGTACCAACAGGCAAATTGCTTGTTTTCTTCTTCTTCTTCTTCTTTTT
TTTTTTTTTTTTAGAAAAAAAGACAAAAACTGATTTTCTTGAAAAAAAAAAATGAACTGTTCTTTCTATA
ATGGCTTTGCCCATTTAAAAAATGTGGCTCTTAAGGGTTCATGAAATGACTGAATATGAGGATACATGTC
CTGTAGAAAGCAAATGCGCCTCATATACTGCCAAAAATAGTGTTAGTTTCATTAATGTGAATTTTCCAGC
ATTCAGTAGTTGTAATGTTAGAAACAATTGCTGGTCAAGTTCAACTTGTTGCTATTGTTTTTAATTTGCA
CAGGAGTAGTATCAGAAATTAGTGTCACTGCTTGTATCTAGCTGAATTTTAAACAACAGAACATTAGTTT
TTTATGTTGGTGCCACCAACTGTAAATGACATAAGTTAGTTATTACAAAACACAGTAATTAGACTGTTGC
AACCATCTAAAACCTTAGGCTTCCAGTCTGTGCTGTTAGTGTTAAGATGTAAAGTGCAATCCTAAGCTAA
CATTATCTGTGCAAGCACCATAGAAACATTTGCATATCTGCATAGATCTTACAACTGTACTCTTTACCTC
CTTGTGATAAAGCTTTGTCTACCTGCAAACACAGTCAAAGGCTACAGCTGCAAACCAAAGCCAACTCTAA
CCATGGCCAAGAGCTCAAGGACAGAAGCAGCCACATGCTTTGGTCAGCCTTCTGTAACTTCAATTAGTAC
AAAGGAACCTTTTCCATGAACTACCTGCTGTTTTCTGATGACCTCTGGGATCTTTTCATTTAGCCCTAAA
CAAAGAAACAAATATGACAAAAACCACAACTAAAAAATGTTAATTCAGTCACAGAGTAATCTTCTGAGGC
CAAAAGTCCATCTAAATGCAATGAAGATTTGCTTTCATTAAAGACAGAGGTGAGGACAAAATC
Sekwencja białka :
forkhead box P2 isoform I [Homo sapiens] - NP_055306.1
>gi|17017963|ref|NP_055306.1| forkhead box P2 isoform I [Homo sapiens]
MMQESATETISNSSMNQNGMSTLSSQLDAGSRDGRSSGDTSSEVSTVELLHLQQQQALQAARQLLLQQQT
SGLKSPKSSDKQRPLQVPVSVAMMTPQVITPQQMQQILQQQVLSPQQLQALLQQQQAVMLQQQQLQEFYK
KQQEQLHLQLLQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQHPGKQAKEQQQQQQQQQQL
AAQQLVFQQQLLQMQQLQQQQHLLSLQRQGLISIPPGQAALPVQSLPQAGLSPAEIQQLWKEVTGVHSME
DNGIKHGGLDLTTNNSSSTTSSNTSKASPPITHHSIVNGQSSVLSARRDSSSHEETGASHTLYGHGVCKW
PGCESICEDFGQFLKHLNNEHALDDRSTAQCRVQMQVVQQLEIQLSKERERLQAMMTHLHMRPSEPKPSP
KPLNLVSSVTMSKNMLETSPQSLPQTPTTPTAPVTPITQGPSVITPASVPNVGAIRRRHSDKYNIPMSSE
IAPNYEFYKNADVRPPFTYATLIRQAIMESSDRQLTLNEIYSWFTRTFAYFRRNAATWKNAVRHNLSLHK
CFVRVENVKGAVWTVDEVEYQKRRSQKITGSPTLVKNIPTSLGYGAALNASLQAALAESSLPLLSNPGLI
NNASSGLLQAVHEDLNGSLDHIDSNGNSSPGCSPQPHIHSIHVKEEPVIAEDEDCPMSLVTTANHSPELE
DDREIEEEPLSEDLE
Informacje dotyczące sekwencji kodującej:
CDS 1..2148
/gene="FOXP2"
/gene_synonym="CAGH44; DKFZp686H1726; SPCH1; TNRC10"
/note="isoform I is encoded by transcript variant 1;
trinucleotide repeat containing 10; forkhead/winged-helix
transcription factor; speech and language disorder 1; CAG
repeat protein 44"
/codon_start=1
/product="forkhead box P2 isoform I"
/protein_id="NP_055306.1"
/db_xref="GI:17017963"
/db_xref="CCDS:CCDS5760.1"
/db_xref="GeneID:93986"
/db_xref="HGNC:13875"
/db_xref="HPRD:05611"
/db_xref="MIM:605317"
/translation="MMQESATETISNSSMNQNGMSTLSSQLDAGSRDGRSSGDTSSEV
STVELLHLQQQQALQAARQLLLQQQTSGLKSPKSSDKQRPLQVPVSVAMMTPQVITPQ
QMQQILQQQVLSPQQLQALLQQQQAVMLQQQQLQEFYKKQQEQLHLQLLQQQQQQQQQ
QQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQHPGKQAKEQQQQQQQQQQLAAQQLVFQ
QQLLQMQQLQQQQHLLSLQRQGLISIPPGQAALPVQSLPQAGLSPAEIQQLWKEVTGV
HSMEDNGIKHGGLDLTTNNSSSTTSSNTSKASPPITHHSIVNGQSSVLSARRDSSSHE
ETGASHTLYGHGVCKWPGCESICEDFGQFLKHLNNEHALDDRSTAQCRVQMQVVQQLE
IQLSKERERLQAMMTHLHMRPSEPKPSPKPLNLVSSVTMSKNMLETSPQSLPQTPTTP
TAPVTPITQGPSVITPASVPNVGAIRRRHSDKYNIPMSSEIAPNYEFYKNADVRPPFT
YATLIRQAIMESSDRQLTLNEIYSWFTRTFAYFRRNAATWKNAVRHNLSLHKCFVRVE
NVKGAVWTVDEVEYQKRRSQKITGSPTLVKNIPTSLGYGAALNASLQAALAESSLPLL
SNPGLINNASSGLLQAVHEDLNGSLDHIDSNGNSSPGCSPQPHIHSIHVKEEPVIAED
EDCPMSLVTTANHSPELEDDREIEEEPLSEDLE"
>gi|149999354:375-2522 Homo sapiens forkhead box P2 (FOXP2), transcript variant 1, mRNA
ATGATGCAGGAATCTGCGACAGAGACAATAAGCAACAGTTCAATGAATCAAAATGGAATGAGCACTCTAA
GCAGCCAATTAGATGCTGGCAGCAGAGATGGAAGATCAAGTGGTGACACCAGCTCTGAAGTAAGCACAGT
AGAACTGCTGCATCTGCAACAACAGCAGGCTCTCCAGGCAGCAAGACAACTTCTTTTACAGCAGCAAACA
AGTGGATTGAAATCTCCTAAGAGCAGTGATAAACAGAGACCACTGCAGGTGCCTGTGTCAGTGGCCATGA
TGACTCCCCAGGTGATCACCCCTCAGCAAATGCAGCAGATCCTTCAGCAACAAGTCCTGTCTCCTCAGCA
GCTACAAGCCCTTCTCCAACAACAGCAGGCTGTCATGCTGCAGCAGCAACAACTACAAGAGTTTTACAAG
AAACAGCAAGAGCAGTTACATCTTCAGCTTTTGCAGCAGCAGCAGCAACAGCAGCAGCAGCAACAACAGC
AGCAACAACAGCAGCAGCAACAACAACAACAACAGCAGCAACAACAGCAGCAGCAGCAGCAACAGCAGCA
GCAGCAGCAACAGCATCCTGGAAAGCAAGCGAAAGAGCAGCAGCAGCAGCAGCAGCAGCAACAGCAATTG
GCAGCCCAGCAGCTTGTCTTCCAGCAGCAGCTTCTCCAGATGCAACAACTCCAGCAGCAGCAGCATCTGC
TCAGCCTTCAGCGTCAGGGACTCATCTCCATTCCACCTGGCCAGGCAGCACTTCCTGTCCAATCGCTGCC
TCAAGCTGGCTTAAGTCCTGCTGAGATTCAGCAGTTATGGAAAGAAGTGACTGGAGTTCACAGTATGGAA
GACAATGGCATTAAACATGGAGGGCTAGACCTCACTACTAACAATTCCTCCTCGACTACCTCCTCCAACA
CTTCCAAAGCATCACCACCAATAACTCATCATTCCATAGTGAATGGACAGTCTTCAGTTCTAAGTGCAAG
ACGAGACAGCTCGTCACATGAGGAGACTGGGGCCTCTCACACTCTCTATGGCCATGGAGTTTGCAAATGG
CCAGGCTGTGAAAGCATTTGTGAAGATTTTGGACAGTTTTTAAAGCACCTTAACAATGAACACGCATTGG
ATGACCGAAGCACTGCTCAGTGTCGAGTGCAAATGCAGGTGGTGCAACAGTTAGAAATACAGCTTTCTAA
AGAACGCGAACGTCTTCAAGCAATGATGACCCACTTGCACATGCGACCCTCAGAGCCCAAACCATCTCCC
AAACCTCTAAATCTGGTGTCTAGTGTCACCATGTCGAAGAATATGTTGGAGACATCCCCACAGAGCTTAC
CTCAAACCCCTACCACACCAACGGCCCCAGTCACCCCGATTACCCAGGGACCCTCAGTAATCACCCCAGC
CAGTGTGCCCAATGTGGGAGCCATACGAAGGCGACATTCAGACAAATACAACATTCCCATGTCATCAGAA
ATTGCCCCAAACTATGAATTTTATAAAAATGCAGATGTCAGACCTCCATTTACTTATGCAACTCTCATAA
GGCAGGCTATCATGGAGTCATCTGACAGGCAGTTAACACTTAATGAAATTTACAGCTGGTTTACACGGAC
ATTTGCTTACTTCAGGCGTAATGCAGCAACTTGGAAGAATGCAGTACGTCATAATCTTAGCCTGCACAAG
TGTTTTGTTCGAGTAGAAAATGTTAAAGGAGCAGTATGGACTGTGGATGAAGTAGAATACCAGAAGCGAA
GGTCACAAAAGATAACAGGAAGTCCAACCTTAGTAAAAAATATACCTACCAGTTTAGGCTATGGAGCAGC
TCTTAATGCCAGTTTGCAGGCTGCCTTGGCAGAGAGCAGTTTACCTTTGCTAAGTAATCCTGGACTGATA
AATAATGCATCCAGTGGCCTACTGCAGGCCGTCCACGAAGACCTCAATGGTTCTCTGGATCACATTGACA
GCAATGGAAACAGTAGTCCGGGCTGCTCACCTCAGCCGCACATACATTCAATCCACGTCAAGGAAGAGCC
AGTGATTGCAGAGGATGAAGACTGCCCAATGTCCTTAGTGACAACAGCTAATCACAGTCCAGAATTAGAA
GACGACAGAGAGATTGAAGAAGAGCCTTTATCTGAAGATCTGGAATGA
Zadanie 4:
Homo sapiens forkhead box P2 (FOXP2), transcript variant 1, mRNA. NM_014491
gene 1..3633
/gene="FOXP2"
/gene_synonym="CAGH44; DKFZp686H1726; SPCH1; TNRC10"
/note="forkhead box P2"
/db_xref="GeneID:93986"
/db_xref="HGNC:13875"
/db_xref="HPRD:05611"
/db_xref="MIM:605317"
exon 1..364
/gene="FOXP2"
/gene_synonym="CAGH44; DKFZp686H1726; SPCH1; TNRC10"
/inference="alignment:Splign"
/number=1
exon 365..542
/gene="FOXP2"
/gene_synonym="CAGH44; DKFZp686H1726; SPCH1; TNRC10"
/inference="alignment:Splign"
/number=2
CDS 375..2522
/gene="FOXP2"
/gene_synonym="CAGH44; DKFZp686H1726; SPCH1; TNRC10"
/note="isoform I is encoded by transcript variant 1;
trinucleotide repeat containing 10; forkhead/winged-helix
transcription factor; speech and language disorder 1; CAG
repeat protein 44"
/codon_start=1
/product="forkhead box P2 isoform I"
/protein_id="NP_055306.1"
/db_xref="GI:17017963"
/db_xref="CCDS:CCDS5760.1"
/db_xref="GeneID:93986"
/db_xref="HGNC:13875"
/db_xref="HPRD:05611"
/db_xref="MIM:605317"
/translation="MMQESATETISNSSMNQNGMSTLSSQLDAGSRDGRSSGDTSSEV
STVELLHLQQQQALQAARQLLLQQQTSGLKSPKSSDKQRPLQVPVSVAMMTPQVITPQ
QMQQILQQQVLSPQQLQALLQQQQAVMLQQQQLQEFYKKQQEQLHLQLLQQQQQQQQQ
QQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQHPGKQAKEQQQQQQQQQQLAAQQLVFQ
QQLLQMQQLQQQQHLLSLQRQGLISIPPGQAALPVQSLPQAGLSPAEIQQLWKEVTGV
HSMEDNGIKHGGLDLTTNNSSSTTSSNTSKASPPITHHSIVNGQSSVLSARRDSSSHE
ETGASHTLYGHGVCKWPGCESICEDFGQFLKHLNNEHALDDRSTAQCRVQMQVVQQLE
IQLSKERERLQAMMTHLHMRPSEPKPSPKPLNLVSSVTMSKNMLETSPQSLPQTPTTP
TAPVTPITQGPSVITPASVPNVGAIRRRHSDKYNIPMSSEIAPNYEFYKNADVRPPFT
YATLIRQAIMESSDRQLTLNEIYSWFTRTFAYFRRNAATWKNAVRHNLSLHKCFVRVE
NVKGAVWTVDEVEYQKRRSQKITGSPTLVKNIPTSLGYGAALNASLQAALAESSLPLL
SNPGLINNASSGLLQAVHEDLNGSLDHIDSNGNSSPGCSPQPHIHSIHVKEEPVIAED
EDCPMSLVTTANHSPELEDDREIEEEPLSEDLE"
exon 543..632
/gene="FOXP2"
/gene_synonym="CAGH44; DKFZp686H1726; SPCH1; TNRC10"
/inference="alignment:Splign"
/number=3
exon 633..770
/gene="FOXP2"
/gene_synonym="CAGH44; DKFZp686H1726; SPCH1; TNRC10"
/inference="alignment:Splign"
/number=6
exon 771..971
/gene="FOXP2"
/gene_synonym="CAGH44; DKFZp686H1726; SPCH1; TNRC10"
/inference="alignment:Splign"
/number=7
exon 972..1149
/gene="FOXP2"
/gene_synonym="CAGH44; DKFZp686H1726; SPCH1; TNRC10"
/inference="alignment:Splign"
/number=8
exon 1150..1363
/gene="FOXP2"
/gene_synonym="CAGH44; DKFZp686H1726; SPCH1; TNRC10"
/inference="alignment:Splign"
/number=9
exon 1364..1468
/gene="FOXP2"
/gene_synonym="CAGH44; DKFZp686H1726; SPCH1; TNRC10"
/inference="alignment:Splign"
/number=10
exon 1469..1556
/gene="FOXP2"
/gene_synonym="CAGH44; DKFZp686H1726; SPCH1; TNRC10"
/inference="alignment:Splign"
/number=11
exon 1557..1640
/gene="FOXP2"
/gene_synonym="CAGH44; DKFZp686H1726; SPCH1; TNRC10"
/inference="alignment:Splign"
/number=12
exon 1641..1842
/gene="FOXP2"
/gene_synonym="CAGH44; DKFZp686H1726; SPCH1; TNRC10"
/inference="alignment:Splign"
/number=13
exon 1843..1919
/gene="FOXP2"
/gene_synonym="CAGH44; DKFZp686H1726; SPCH1; TNRC10"
/inference="alignment:Splign"
/number=14
exon 1920..2021
/gene="FOXP2"
/gene_synonym="CAGH44; DKFZp686H1726; SPCH1; TNRC10"
/inference="alignment:Splign"
/number=15
exon 2022..2143
/gene="FOXP2"
/gene_synonym="CAGH44; DKFZp686H1726; SPCH1; TNRC10"
/inference="alignment:Splign"
/number=16
exon 2144..2213
/gene="FOXP2"
/gene_synonym="CAGH44; DKFZp686H1726; SPCH1; TNRC10"
/inference="alignment:Splign"
/number=17
exon 2214..2377
/gene="FOXP2"
/gene_synonym="CAGH44; DKFZp686H1726; SPCH1; TNRC10"
/inference="alignment:Splign"
/number=18
exon 2378..3633
/gene="FOXP2"
/gene_synonym="CAGH44; DKFZp686H1726; SPCH1; TNRC10"
/inference="alignment:Splign"
/number=19
STS 2505..2729
/gene="FOXP2"
/gene_synonym="CAGH44; DKFZp686H1726; SPCH1; TNRC10"
/standard_name="REN103420"
/db_xref="UniSTS:428217"
STS 2693..2957
/gene="FOXP2"
/gene_synonym="CAGH44; DKFZp686H1726; SPCH1; TNRC10"
/standard_name="REN103421"
/db_xref="UniSTS:428218"
STS 2924..3176
/gene="FOXP2"
/gene_synonym="CAGH44; DKFZp686H1726; SPCH1; TNRC10"
/standard_name="REN103422"
/db_xref="UniSTS:428219"
STS 3174..3423
/gene="FOXP2"
/gene_synonym="CAGH44; DKFZp686H1726; SPCH1; TNRC10"
/standard_name="REN103423"
/db_xref="UniSTS:428220"
Zadanie 5 :
Pan troglodytes forkhead box P2 (FOXP2), mRNA - NM_001009020.1
Mus musculus forkhead box P2 (Foxp2), transcript variant 2, mRNA - NM_212435.1
Pan paniscus FOXP2 protein (FOXP2) mRNA, complete cds - AY143179.1
Zadanie 6:
Primer pair 1
|
Sequence (5'->3') |
Strand on template |
Length |
Start |
Stop |
Tm |
GC% |
Forward primer |
CACCAACGGCCCCAGTCACC |
Plus |
20 |
1346 |
1365 |
59.90 |
70.00% |
Reverse primer |
GGTCTTCGTGGACGGCCTGC |
Minus |
20 |
1933 |
1914 |
60.04 |
70.00% |
Internal oligo |
|
Plus |
|
|
|
|
|
Product length |
588 |
|
|||||
Products on potentially unintended templates
>NM_014491.2 Homo sapiens forkhead box P2 (FOXP2), transcript variant 1, mRNA
product length = 588
Forward primer 1 CACCAACGGCCCCAGTCACC 20
Template 1720 .................... 1739
Reverse primer 1 GGTCTTCGTGGACGGCCTGC 20
Template 2307 .................... 2288
>NM_148899.2 Homo sapiens forkhead box P2 (FOXP2), transcript variant 3, mRNA
product length = 588
Forward primer 1 CACCAACGGCCCCAGTCACC 20
Template 1778 .................... 1797
Reverse primer 1 GGTCTTCGTGGACGGCCTGC 20
Template 2365 .................... 2346
>NM_148898.2 Homo sapiens forkhead box P2 (FOXP2), transcript variant 2, mRNA
product length = 588
Forward primer 1 CACCAACGGCCCCAGTCACC 20
Template 1795 .................... 1814
Reverse primer 1 GGTCTTCGTGGACGGCCTGC 20
Template 2382 .................... 2363
Primer pair 2
|
Sequence (5'->3') |
Strand on template |
Length |
Start |
Stop |
Tm |
GC% |
Forward primer |
CCACACCAACGGCCCCAGTC |
Plus |
20 |
1343 |
1362 |
59.90 |
70.00% |
Reverse primer |
GGTCTTCGTGGACGGCCTGC |
Minus |
20 |
1933 |
1914 |
60.04 |
70.00% |
Internal oligo |
|
Plus |
|
|
|
|
|
Product length |
591 |
|
|||||
Products on potentially unintended templates
>NM_014491.2 Homo sapiens forkhead box P2 (FOXP2), transcript variant 1, mRNA
product length = 591
Forward primer 1 CCACACCAACGGCCCCAGTC 20
Template 1717 .................... 1736
Reverse primer 1 GGTCTTCGTGGACGGCCTGC 20
Template 2307 .................... 2288
>NM_148899.2 Homo sapiens forkhead box P2 (FOXP2), transcript variant 3, mRNA
product length = 591
Forward primer 1 CCACACCAACGGCCCCAGTC 20
Template 1775 .................... 1794
Reverse primer 1 GGTCTTCGTGGACGGCCTGC 20
Template 2365 .................... 2346
>NM_148898.2 Homo sapiens forkhead box P2 (FOXP2), transcript variant 2, mRNA
product length = 591
Forward primer 1 CCACACCAACGGCCCCAGTC 20
Template 1792 .................... 1811
Reverse primer 1 GGTCTTCGTGGACGGCCTGC 20
Template 2382 .................... 2363
Primer pair 3
|
Sequence (5'->3') |
Strand on template |
Length |
Start |
Stop |
Tm |
GC% |
Forward primer |
AGCAGGCTCTCCAGGCAGCA |
Plus |
20 |
164 |
183 |
60.18 |
65.00% |
Reverse primer |
GACTGGGGCCGTTGGTGTGG |
Minus |
20 |
1362 |
1343 |
59.90 |
70.00% |
Internal oligo |
|
Plus |
|
|
|
|
|
Product length |
1199 |
|
|||||
Products on potentially unintended templates
>NM_014491.2 Homo sapiens forkhead box P2 (FOXP2), transcript variant 1, mRNA
product length = 1199
Forward primer 1 AGCAGGCTCTCCAGGCAGCA 20
Template 538 .................... 557
Reverse primer 1 GACTGGGGCCGTTGGTGTGG 20
Template 1736 .................... 1717
>NM_148899.2 Homo sapiens forkhead box P2 (FOXP2), transcript variant 3, mRNA
product length = 1257
Forward primer 1 AGCAGGCTCTCCAGGCAGCA 20
Template 538 .................... 557
Reverse primer 1 GACTGGGGCCGTTGGTGTGG 20
Template 1794 .................... 1775
>NM_148898.2 Homo sapiens forkhead box P2 (FOXP2), transcript variant 2, mRNA
product length = 1274
Forward primer 1 AGCAGGCTCTCCAGGCAGCA 20
Template 538 .................... 557
Reverse primer 1 GACTGGGGCCGTTGGTGTGG 20
Template 1811 .................... 1792
Primer pair 4
|
Sequence (5'->3') |
Strand on template |
Length |
Start |
Stop |
Tm |
GC% |
Forward primer |
AGCAGGCTCTCCAGGCAGCA |
Plus |
20 |
164 |
183 |
60.18 |
65.00% |
Reverse primer |
GGTGACTGGGGCCGTTGGTG |
Minus |
20 |
1365 |
1346 |
59.90 |
70.00% |
Internal oligo |
|
Plus |
|
|
|
|
|
Product length |
1202 |
|
|||||
Products on potentially unintended templates
>NM_014491.2 Homo sapiens forkhead box P2 (FOXP2), transcript variant 1, mRNA
product length = 1202
Forward primer 1 AGCAGGCTCTCCAGGCAGCA 20
Template 538 .................... 557
Reverse primer 1 GGTGACTGGGGCCGTTGGTG 20
Template 1739 .................... 1720
>NM_148899.2 Homo sapiens forkhead box P2 (FOXP2), transcript variant 3, mRNA
product length = 1260
Forward primer 1 AGCAGGCTCTCCAGGCAGCA 20
Template 538 .................... 557
Reverse primer 1 GGTGACTGGGGCCGTTGGTG 20
Template 1797 .................... 1778
>NM_148898.2 Homo sapiens forkhead box P2 (FOXP2), transcript variant 2, mRNA
product length = 1277
Forward primer 1 AGCAGGCTCTCCAGGCAGCA 20
Template 538 .................... 557
Reverse primer 1 GGTGACTGGGGCCGTTGGTG 20
Template 1814 .................... 1795
Primer pair 5
|
Sequence (5'->3') |
Strand on template |
Length |
Start |
Stop |
Tm |
GC% |
Forward primer |
CAGCAGGCTCTCCAGGCAGC |
Plus |
20 |
163 |
182 |
59.76 |
70.00% |
Reverse primer |
GACTGGGGCCGTTGGTGTGG |
Minus |
20 |
1362 |
1343 |
59.90 |
70.00% |
Internal oligo |
|
Plus |
|
|
|
|
|
Product length |
1200 |
|
|||||
Products on potentially unintended templates
>NM_014491.2 Homo sapiens forkhead box P2 (FOXP2), transcript variant 1, mRNA
product length = 1200
Forward primer 1 CAGCAGGCTCTCCAGGCAGC 20
Template 537 .................... 556
Reverse primer 1 GACTGGGGCCGTTGGTGTGG 20
Template 1736 .................... 1717
>NM_148899.2 Homo sapiens forkhead box P2 (FOXP2), transcript variant 3, mRNA
product length = 1258
Forward primer 1 CAGCAGGCTCTCCAGGCAGC 20
Template 537 .................... 556
Reverse primer 1 GACTGGGGCCGTTGGTGTGG 20
Template 1794 .................... 1775
product length = 747
Forward primer 1 CAGCAGGCTCTCCAGGCAGC 20
Template 1048 .....ACAG.AATT...... 1067
Reverse primer 1 GACTGGGGCCGTTGGTGTGG 20
Template 1794 .................... 1775
>NM_148898.2 Homo sapiens forkhead box P2 (FOXP2), transcript variant 2, mRNA
product length = 1275
Forward primer 1 CAGCAGGCTCTCCAGGCAGC 20
Template 537 .................... 556
Reverse primer 1 GACTGGGGCCGTTGGTGTGG 20
Template 1811 .................... 1792
product length = 747
Forward primer 1 CAGCAGGCTCTCCAGGCAGC 20
Template 1065 .....ACAG.AATT...... 1084
Reverse primer 1 GACTGGGGCCGTTGGTGTGG 20
Template 1811 .................... 1792
Primer pair 6
|
Sequence (5'->3') |
Strand on template |
Length |
Start |
Stop |
Tm |
GC% |
Forward primer |
ACCACACCAACGGCCCCAGT |
Plus |
20 |
1342 |
1361 |
60.61 |
65.00% |
Reverse primer |
GGTCTTCGTGGACGGCCTGC |
Minus |
20 |
1933 |
1914 |
60.04 |
70.00% |
Internal oligo |
|
Plus |
|
|
|
|
|
Product length |
592 |
|
|||||
>NM_014491.2 Homo sapiens forkhead box P2 (FOXP2), transcript variant 1, mRNA
product length = 592
Forward primer 1 ACCACACCAACGGCCCCAGT 20
Template 1716 .................... 1735
Reverse primer 1 GGTCTTCGTGGACGGCCTGC 20
Template 2307 .................... 2288
>NM_148899.2 Homo sapiens forkhead box P2 (FOXP2), transcript variant 3, mRNA
product length = 592
Forward primer 1 ACCACACCAACGGCCCCAGT 20
Template 1774 .................... 1793
Reverse primer 1 GGTCTTCGTGGACGGCCTGC 20
Template 2365 .................... 2346
>NM_148898.2 Homo sapiens forkhead box P2 (FOXP2), transcript variant 2, mRNA
product length = 592
Forward primer 1 ACCACACCAACGGCCCCAGT 20
Template 1791 .................... 1810
Reverse primer 1 GGTCTTCGTGGACGGCCTGC 20
Template 2382 .................... 2363
Primer pair 7
|
Sequence (5'->3') |
Strand on template |
Length |
Start |
Stop |
Tm |
GC% |
Forward primer |
ACCCCAGCCAGTGTGCCCAA |
Plus |
20 |
1393 |
1412 |
60.61 |
65.00% |
Reverse primer |
GGTCTTCGTGGACGGCCTGC |
Minus |
20 |
1933 |
1914 |
60.04 |
70.00% |
Internal oligo |
|
Plus |
|
|
|
|
|
Product length |
541 |
|
|||||
Products on potentially unintended templates
>NM_014491.2 Homo sapiens forkhead box P2 (FOXP2), transcript variant 1, mRNA
product length = 541
Forward primer 1 ACCCCAGCCAGTGTGCCCAA 20
Template 1767 .................... 1786
Reverse primer 1 GGTCTTCGTGGACGGCCTGC 20
Template 2307 .................... 2288
>NM_148899.2 Homo sapiens forkhead box P2 (FOXP2), transcript variant 3, mRNA
product length = 541
Forward primer 1 ACCCCAGCCAGTGTGCCCAA 20
Template 1825 .................... 1844
Reverse primer 1 GGTCTTCGTGGACGGCCTGC 20
Template 2365 .................... 2346
>NM_148898.2 Homo sapiens forkhead box P2 (FOXP2), transcript variant 2, mRNA
product length = 541
Forward primer 1 ACCCCAGCCAGTGTGCCCAA 20
Template 1842 .................... 1861
Reverse primer 1 GGTCTTCGTGGACGGCCTGC 20
Template 2382 .................... 2363
Primer pair 8
|
Sequence (5'->3') |
Strand on template |
Length |
Start |
Stop |
Tm |
GC% |
Forward primer |
CCTACCACACCAACGGCCCC |
Plus |
20 |
1339 |
1358 |
59.34 |
70.00% |
Reverse primer |
GGTCTTCGTGGACGGCCTGC |
Minus |
20 |
1933 |
1914 |
60.04 |
70.00% |
Internal oligo |
|
Plus |
|
|
|
|
|
Product length |
595 |
|
|||||
Products on potentially unintended templates
>NM_014491.2 Homo sapiens forkhead box P2 (FOXP2), transcript variant 1, mRNA
product length = 595
Forward primer 1 CCTACCACACCAACGGCCCC 20
Template 1713 .................... 1732
Reverse primer 1 GGTCTTCGTGGACGGCCTGC 20
Template 2307 .................... 2288
>NM_148899.2 Homo sapiens forkhead box P2 (FOXP2), transcript variant 3, mRNA
product length = 595
Forward primer 1 CCTACCACACCAACGGCCCC 20
Template 1771 .................... 1790
Reverse primer 1 GGTCTTCGTGGACGGCCTGC 20
Template 2365 .................... 2346
>NM_148898.2 Homo sapiens forkhead box P2 (FOXP2), transcript variant 2, mRNA
product length = 595
Forward primer 1 CCTACCACACCAACGGCCCC 20
Template 1788 .................... 1807
Reverse primer 1 GGTCTTCGTGGACGGCCTGC 20
Template 2382 .................... 2363
Primer pair 9
|
Sequence (5'->3') |
Strand on template |
Length |
Start |
Stop |
Tm |
GC% |
Forward primer |
CCCTACCACACCAACGGCCC |
Plus |
20 |
1338 |
1357 |
59.34 |
70.00% |
Reverse primer |
GGTCTTCGTGGACGGCCTGC |
Minus |
20 |
1933 |
1914 |
60.04 |
70.00% |
Internal oligo |
|
Plus |
|
|
|
|
|
Product length |
596 |
|
|||||
Products on potentially unintended templates
>NM_014491.2 Homo sapiens forkhead box P2 (FOXP2), transcript variant 1, mRNA
product length = 596
Forward primer 1 CCCTACCACACCAACGGCCC 20
Template 1712 .................... 1731
Reverse primer 1 GGTCTTCGTGGACGGCCTGC 20
Template 2307 .................... 2288
>NM_148899.2 Homo sapiens forkhead box P2 (FOXP2), transcript variant 3, mRNA
product length = 596
Forward primer 1 CCCTACCACACCAACGGCCC 20
Template 1770 .................... 1789
Reverse primer 1 GGTCTTCGTGGACGGCCTGC 20
Template 2365 .................... 2346
>NM_148898.2 Homo sapiens forkhead box P2 (FOXP2), transcript variant 2, mRNA
product length = 596
Forward primer 1 CCCTACCACACCAACGGCCC 20
Template 1787 .................... 1806
Reverse primer 1 GGTCTTCGTGGACGGCCTGC 20
Template 2382 .................... 2363
Primer pair 10
|
Sequence (5'->3') |
Strand on template |
Length |
Start |
Stop |
Tm |
GC% |
Forward primer |
GCAGGCTCTCCAGGCAGCAA |
Plus |
20 |
165 |
184 |
59.34 |
65.00% |
Reverse primer |
GACTGGGGCCGTTGGTGTGG |
Minus |
20 |
1362 |
1343 |
59.90 |
70.00% |
Internal oligo |
|
Plus |
|
|
|
|
|
Product length |
1198 |
|
|||||
Products on potentially unintended templates
>NM_014491.2 Homo sapiens forkhead box P2 (FOXP2), transcript variant 1, mRNA
product length = 1198
Forward primer 1 GCAGGCTCTCCAGGCAGCAA 20
Template 539 .................... 558
Reverse primer 1 GACTGGGGCCGTTGGTGTGG 20
Template 1736 .................... 1717
>NM_148899.2 Homo sapiens forkhead box P2 (FOXP2), transcript variant 3, mRNA
product length = 1256
Forward primer 1 GCAGGCTCTCCAGGCAGCAA 20
Template 539 .................... 558
Reverse primer 1 GACTGGGGCCGTTGGTGTGG 20
Template 1794 .................... 1775
>NM_148898.2 Homo sapiens forkhead box P2 (FOXP2), transcript variant 2, mRNA
product length = 1273
Forward primer 1 GCAGGCTCTCCAGGCAGCAA 20
Template 539 .................... 558
Reverse primer 1 GACTGGGGCCGTTGGTGTGG 20
Template 1811 .................... 1792
Zadanie 7:
Konserwatywne domeny występujące w genie FOXP2:
cd00059, FH, Forkhead (FH), also known as a "winged helix". FH is named for the Drosophila fork head protein, a transcription factor which promotes terminal rather than segmental development. This family of transcription factor domains, which bind to B-DNA as monomers, are also found in the Hepatocyte nuclear factor (HNF) proteins, which provide tissue-specific gene regulation. The structure contains 2 flexible loops or "wings" in the C-terminal region, hence the term winged helix.
CD Length: 78 Bit Score: 110.22 E-value: 9e-26
Zadanie 8:
Numer dostępu do struktury białka FOXP2 - MMDB ID: 52442
PDB ID: 2A07
Funkcjonalne białko FOXP2 składa się z 6 łańcuchów : F,G,H,I,J,K.
Zadanie 9:
Markery STS znajdujące się najbliżej genu:
UniSTS:449911 tSG611520
Homo sapiens chromosome 7, locus FOXP2
Canis familiaris chromosome 14, locus FOXP2
Equus caballus chromosome 4, locus LOC100055896
Gallus gallus chromosome 1, locus FOXP2
Macaca mulatta chromosome 3, locus FOXP2
Mus musculus chromosome 6, locus Foxp2
Pan troglodytes chromosome 7, locus FOXP2
Taeniopygia guttata chromosome 1A
UniSTS:36980 D7S1419
Homo sapiens chromosome 7, locus FOXP2
Pan troglodytes chromosome 7, locus FOXP2
UniSTS:449912 stSG611521
Homo sapiens chromosome 7
Bos taurus chromosome 4
Canis familiaris chromosome 14
Equus caballus chromosome 4
Gallus gallus chromosome 1
Macaca mulatta chromosome 3
Monodelphis domestica chromosome 8
Mus musculus chromosome 6, locus Foxp2
Pan troglodytes chromosome 7
Taeniopygia guttata chromosome 1A
Xenopus tropicalis locus foxp2
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