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Pathophysiological Effects of Vibrio cholerae and Enterotoxigenic Escherichia coli and Their Exotoxins on Eucaryotic Cells

KAREN L. RICHARDS and STEVEN D. DOUGLAS

Departmenis of Microbiology and Medicine, University of Minnesota Medical School. Minneapolis, Minnesota 55455

INTRODUCTION............................................................................................592

PATHOPHYSIOLOGY...................................................................................593

Eliology................................................................................................................593

Factors in Pathogenesis.......................................................................................593

Gcnctic and Physiological Basis for Toxin Production.......................................593

THE TOXINS...................................................................................................594

Structure...............................................................................................................594

Antigenic Rclatcdncss of Toxin$.........................................................................594

Binding Sitc.........................................................................................................595

Action of Toxins on Adenylate Cyclase..............................................................596

Solubilized Adenylate Cyclase and Toxins.........................................................597

Cofactors Rcquircd forToxin Activity................................................................597

Involvement of Guanosine 5-Monophosphate (GMP).......................................597

Effect of Increased Intracellular Cyclic Adenosine 3’.5’-Monophosphate..........598

Variability in Activation of Adenylate Cyclase...................................................598

Similarities to Glycoprotcin Hormoncs and Othcr Bacterial Toxins...................598

Vaccines and Immunity.......................................................................................599

ENTEROTOXINS IN IN VIVO SYSTEMS...................................................600

Wholc-Animal Models........................................................................................600

llcal Loop Assay..................................................................................................600

Skin Permeability Assay......................................................................................601

ENTEROTOXINS IN IN VITRO SYSTEMS.................................................601

Erythrocyte Ghosts...............................................................................................601

Adrcnal Cells.......................................................................................................602

Isolated Fat Cells.................................................................................................603

Lymphocyte and Lymphoid Celi Lines and the Immune Response....................603

Fibroblasts............................................................................................................604

Othcr Celi Systems..............................................................................................604

DISCUSSION...................................................................................................605

CONCLUSIONS..............................................................................................606

LITERATURĘ CITED.....................................................................................606


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