081

081

)

)

attB-PCR product

ICTTGTACAAAGTGGTCCCC

ITTTCACCAGGGG

pDONR™221 or vector—-n₇₅-ccaactti|gtacaaaaaagctgaac-n_1G0------— n₁₀₀-gttcagcttt]cttgtacaaagttgg-n₇₅--

pDONR^,M/ZeO yector—N₇S-GGTTGAAACATGTT1TTTCGACTTG-N_1qo--------* N ^ ₀ q ~CAAGTCGAAAGAACATg]tTTC AACC>N_{7 5} - •

I______I L—-1

-vector

-vector

a«P1

attP2

BP Clonase™

Entry clone

vector---N₇₅-CCAACTTT]

vector—n₇₅-ggttgaaacatgttt;

attU

'; 5 jtt

w^xmm

------AęęCAGCTTTCTTGTACAAAGTTGG-N

jBM IBHSIHMn

-----------_T,

---- ACGCAbG1TTG T T GIAL

OT

15'

\CATGTTTCAACC-N ₇5- -

-vector

-vector

atłL2

By-product

GGGGACAAGTTT GTACAAAAAAGCTGAAC-N₁00----------~Nj _Q o ~^GTTCAGCTTT cttgtacaaagtggtcccc

,, ccciB*Orn'^ -_

cccctgttcaaacatgttitttcgacttg-n₁₀₀-----------^Nioo^{_CAAGTCGAAAGAACATGTTTCACCAGGGG}

affR2

att R1

attBl CNNNTTTGTACAAAAAANNNG

attBl CNNNTTTCTTGTACAAANNNG

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