23 Interferencja RNA

Trigger dsRNA

1 Dicer

^w

, f| | | | | | | 1 I ³‘, P | | | | | | | 1 I ³‘, P | | | | | | | I 1 ³' 3’N II II II l.p 3'^-Ll i i i i Up 3' 1 1 I I I I I I lip

2

RISC

Pilili' -<

PassengGr RNA

'I I I I I I I I I I I I I ⁺ 'I I I I I I I I I I I I I I I I I Cleaved mRNA

Figurę 31-72 A mechanism for RNA interference (RNAi). Sec the tcxt for dctails. ATP is reąuired for Diccr-catalyzcd cleavage of RNA and for RlSC-associated helicasc unwinding of double-strandcd RNA. Depending on the species. the mRNA may not be completely dcgraded.