3582277070

November20, 2016 Biochemistry Exam 3

(1) Giventheequation E +S=ES=E +P

Label and number the 3 ratę constants.

Write Km in terms of the 3 ratę constants. Then, derive the units of Km. (6points)

(2) Given are two mechanisms for a well-known enzyme.

^HSc

acid ^enzyme

_Ccr^o

,-OH /

A-O ,

-OR

o

,-OH

A-C

enzyme

O^O

HO

,H

HOj.

ROH

OH

V^e^ase

enzyme

°y°^h

enzyme

enzyme

_C<A>

_r0H j*

acidtase

enzyme

nucieophitó

AcHN > O

r

enzyme

+ H;0

ROH

-OH

enzyme

0^0

I

H

"rfoX^-OH

AcHN

°Y°^e

enzyme

glycosyl enzyme mtermediate

Name the enzyme. What are the mechanisms here? Explain the reasons behind your answer.

(6points)