5469092039

Analytical application of immobilized enzymes. Current trends m enzymatic techniąues. Using enzymes in molecular analysis and biology.

•    Laboratory - the contents: General properties of enzymes. Enzyme isolation and determination. Enzyme stabilization methods. Kinetics of enzymatic reactions. Methods for determination of Vmax and Km. Identifying type of kinetics and enzyme activity. Methods of distinction between competitive and non-competitive inhibition. Qualitative studies of some enzymes. Methods for activity determination of some enzymes. Enzyme immobilization methods.

•    Proj ect - the contents:

•    Basic literaturę:

1.    Kłyszejko-Stefanowicz L.(red ): Ćwiczenia z biochemii, PWN, Warszawa, 2003.

2.    Mejbaum-Katzenellenbogen W., Mochnacka I.: Kurs praktyczny biochemii, PWN, Warszawa, 1966.

3.    Karpińska-Smulikowska J., Lejczak B., Pawlaczyk-Szpilowa M.: Ćwiczenia laboratoryjne z Biochemii. Skrypt PWr, Wrocław 1979.

4.    Kafarski P., Lejczak B.: Chemia bioorganiczna. Wydawnictwo Naukowe PWN, Warszawa 1994

5.    Witwicki J., Ardelta W.: Elementy enzymologii. PWN, Warszawa 2000.

•    Additional literaturę:

1.    Alberts B. i inni: Podstawy biologii komórki. PWN, Warszawa 1999.

2.    Stryer L.: Biochemia. PWN, Warszawa 1997.

•    Conditions of the course acceptance/credition: Attendance, positive marks received for tests, prepared and submitted presentation and reports from laboratory works.

- depending on a system of studies