8107620376

Folia Medica Lodziensia, 2011, 38:201-213

Potential prognostic importance of minichromosome maintenance 2 (MCM2) protein in chosen rare solid tumors in children

Potencjalna wartość prognostyczna białka MCM2 w wybranej grupie rzadkich guzów litych u dzieci

KATARZYNA TARAN¹'², ANNA SITKIEWICZ²,

EWA ANDRZEJEWSKA², JÓZEF KOBOS³

'Department of Pathology, Medical University of Lodź

Department of Oncology and Pediatrie Surgery, Konopnicka Memoriał Hospital, Medical University of Lodź Department of Pathology of the Age of Development, Konopnicka Memoriał Hospital, Medical University of Lodź

Abstract

Introduction: Pediatrie solid tumors represent a variety of morphologic pictures and clinical behavior. Unexpected results of the performed treatment point that the biology of those entities is still not completely known. In nephron-blastoma and neuroblastoma group of tumors - the commonest extracranial pediatrie solid tumors, the list of prognostic factors is numerous and most widely accepted. Unfortunately, among rarely appearing entities, one of the main clinical problems is still the estimation of prognosis in individual cases. The aim of our study was to examine the expression of minichromosome maintenance 2 (MCM2) by immunohistochemistry in a chosen group of rare solid children tumors and to estimate its possible prognostic value by the comparison with the main accepted prognostic markers for this group - histological type of tumor.

Materials and methods: We selected study 20 formalin-fixed and paraffin-embedded tumor tissue sections of pediatrie solid tumors: 8 cases of rhabdomyosarcoma, 3 EWS/PNETs, 2 cases of fibromatosis and 7 cases of other sarcomas. Ali those tumor tissue samples were used for immunohisto-chemical research with the use of minichromosome maintenance 2 (MCM2) antibody produced by Thermo Scientific Comp. Labeling index (LI) of the MCM2 protein positive cells was scored in 10 HPF in every case and 1000 tumor cells were examined.

Results: Our studies showed that MCM2 expression appeared to correlate with the prognosis based on the histology of the tumor. We found the highest MCM2 expression (90%) among cases with unfavorable histology and unfavorable course of disease.

Conclusions: MCM2 is a promising marker in prognostication of solid tumors in children and further studies on a morę numerous group are necessary in order to assess the clinical value of MCM2 protein in this group of tumors.

Key words: MCM2, solid tumors, childhood, prognosis