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fn generał, che number of P. ptmtrans recovcrcd from luceme roots was not significamly influcnced by treatment of roots either with crude fungaienzyme prcpurations or with purified polygalacturonase or cellulasc.

0706 -HOWELL, R. K. & KRUSBERG, L. R., 1966. “Changes in conccmrations of nitrogeo and free and bound amino acids in alfaifa and pca infected by Ditylenchus dipsaciPhyio-palhology, 56 (10), 1170-1177.

ToiaJ niirogen and frce and bound amino-acids in luceme and pea seedling shooi tissues infeaed with Ditylenchus dipsaci wcre compared with those in non-infeaed tissues at 7, 14, 21 and 28 days aficr inoculation of germinating secds. At 28 days almost 2 5 times as many nematodes were obtained from pca seedling sboots as from luceme shoots. Infeaed tissues contained slightly morę dry maner than non-infeaed tissues. Dry weight of all tissues increased with each successive harvest and varied from 8 0 to 9-7%. Total nitrogen and total frce and bound amino-acid comcois of luceme and pea tissues also increased with each successive harvest; the increases were much greater in infeaed than iD non-infccted tissues. Most free amino-acids in luceme and pea shoots increased morę rapidly in infected than in healtby tissues with succcssive harvests. In infected lucernę tissues, the amino-acids that increased most significamly, based on the ratio of galled to hcalthy tissue, were arginine, asparagine, threonine and tryptophan; in pea, arginine, asparagine, serine and tryptophan increased most significantly. The galled to hraJthy tissue ratio of glutamic acid content of both plant species was grcaiest at 7 days. Tłiercafter. this amino-acid increased morę rapidly in non-infected than in infected tissues, although at 28 days the ratios were stiU greater than one. The paticms of bound amino-acids in infeaed 0707 CRIFFIN, C.D., 1966. ^,rEffect of environiDent on assoclatlon of Ditylenchus dipsaci to alfaifa.*' (Abstract.J Phytopa-thology, 56 (8), 879.

*0708 SRIVASTAVA, A.S.; UPADHYAY, K.D.; SINGH, G. Effect of root-knot nematode, Meloldogyrie javanica on grata crop. Indian Journal of Nematology (1974 publ. 1975) 4 (2) 248-251 (En) Div. of Entono1., U.P. Inst. of Agrlc. Sciences, Kanpur, India.

A    continuous    reductlon    in    length    and

weight of roots and shoots of gram (Cicer arietlnurn) was obtained with an lncrease in tKe    Ievel of    lnoculum    of    larvae of

Meloidogyne Jayanlca from 10 to 10,000/500 g soilT Hovever, slgnlficant reductlon compared with the unlnoculated control was found only with 100 or morę larvae/500 g soli. The majtlmum reductlon was observed with the 10,000 larval lnoculun.

0709—JOHNSON, P. W. & FUSHTEY, S. G., 1967. “The biology of the oat cyst nemacode Heterodera auenae in Canada. II. Nematode development and related anacomical changes in roots of oats and corn.” Nematologica, Year 1966, 12 (4), 630-636. (German sumraary p. 635.J

Scudies on che developmenc of Heterodera antnat and on relaccd anatomical changes within roots of Avena salina and Zea mays (variery Pride 5) showed that the latter is an unsuitable host for the develop-ment of H. anenae. Nematodes readily entered roots of both piants but their development in Z.

mays was inhibited by a neerotie tissue reaaion and female nematodes failed to produce viable c/sts. Nematode development and related histologicaJ changes within invaded rooc tissues arc described for each host and differences in host-parasite relationships are discussed.

0710 -HA\VN, E. J. & HANNA, M. R., 1967. ‘'Influence of stem aemacode infestation on baaeriał wilt reaaion and forage yield of alfaifa yarieties.” Can. J. PI. Sci., 47 (2), 203-208.

After giasshouse experiments in uhich Ditylenchus dipsaci carried Corynebacterium insidiosum inco the ctowo buds of luceme (see Helminih. Abstr., 3*1, No. 1020] field cxperimencs were done with luceme grown under irrigacion in southem Alberta, Canada, to exnmine (i) the effects of stem nematode on che susceptibilicy of luceme to baaeriał wilc and (ii) the effea of stem nematode on hay production. In expcriment (i), luceme varieties Grimm (sus-ceptible to D. dipsaci and C. insidiosum), Vemal (partially resistant to D. dipsaci, very resistant to C. insidiosum), Beaver (susccptible to D. dipsaci, very resistant to C. insidiosum) and Lahoncan (resistant to D. dipsaci and C. insidiosum) were inocuiaced with both pathogens in a factorial design and rated foc nematode infestation and baaeriał wilt. In enperimcnc (ii), lucernę varictie Grimm, Vernal, Beaver, Lahontan, Lądek (sus-ceptible to D. dipsaci, partially resistant to C. insidiosum) and Talent (resistant to D. dipsaci, susceptible to C. insidiosum) were inoeulated with D. dipsaci and rated for winter injury and yield. Grimm became infeaed with bacterial wilt in all treatments but when bacteria and nematodes were both inoeulated the incideDce of baaeriał wilt increased. The incidence of bacterial wilt in Vernal remained smali in spite of moderate infestation with nematodes. The incidcnce of bacterial wilt in Bcaver was increased by the nematodes and was equal to that of Grimm when both pathogens were inoeulated. Lahoman retained its resistance to both pathogens. In cxpcriment (ii), there were no significam losses in the first season but all cxcepi Talent and Lahontan suflered winter injury and yielded less in the following season. Ycrnal recovered better than the other varieties but similar trends were established in the 3rd season. Tbesc exj>eriments show the need for a cold-hardy variery of lucernę resistant to both D. dipsaci and C. insidiosum for the irrigated areas of Southern Albena.

0711 -RAINA, R., 1966.    “Longidorus reneyii

sp.nov. (Nematoda: Longidocidae) from Srinagar, Kashmir.” Indian J. Ent., 28 (4), 438-441

Longidorus reneyii n.sp. is described from 5 female specimens taken from soil around roots of Zea mays. It is 21 to 2-6 mm. loog, has a spear 50 to 58 p long and a spear extension 30 to 36 p loag. The guiding ring is 2\ head widths from che ancerior

0712 -JENKINS, W. R. & MAŁEK, R. B., 1966. “Influence of nematodes on absorption and accumulation of nutrients in verch.” Soil Sci., 101 (1), 46-49.

Vida nillosa was grown in pots and inoeulated with Meloidogyne hopla, Trichodorus christici, Criccmemoides curuaium or Scuiellonema brachyurum. The tops of the piants were cropped and analysed for nitrogen, phosphorus, potassium, calcium and sodium 30, 60 and 90 days after inoculation; the roots were analysed 90 days afier inoculation. 184 Changes in plam nutrients varied with the nematode