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VolatiIe Fraction from Sagę (Salvia L.) Species 5

Accelerated Solvent Extraction (ASE)

Extraction was performed in a Dionex (Sunnyvale, CA, USA) model ASE 200 accelerated solvent extraction unit. The operating conditions were: purge time, 60 s; extracting solvent, petroleum ether; static cycles, 3; oven temperaturę, 105°C; working pressure, 6.67 Mpa; oven heat-up time, 5 min; static time, 10 min; flush volume, 100%. Petroleum ether was used for isola-tion of the volatile compounds because it has been recommended elsewhere for this purpose [13].

Each extract obtained by ASE was kept frozen for 24 h at -6°C to sepa-rate the ballast constituents. Extracts were then filtered through an Anotop syringe filter with aluminium oxide as adsorbent and 1-pL samples were analyzed by GC-MS.

Identification of the Volatile Compounds

Identification of individual volatile compounds was based on comparison of their mass spectra with those from standards and spectra available from the NIST (National Institute of Standards and Technology, Gaithersburg, MD, USA) library. The retention times of peaks obtained from the Salwa samples were also compared with those of compounds known to be present in the essential oils of other plants. For this purpose we used pine oil, pep-permint oil, eucalyptus oil, and juniper oil (manufactured by Apotheca Pacis, Rybnik, Poland) as the volatile standards.

Results and Discussion

The first step in our study was to compare four different techniques for iso-lating volatde compounds from the crude plant materiał. This comparison was performed using results obtained from sagę samples collected in 2007 ordy. The results obtained are summarized in Tobie 1 and Figs 1-5.