1254394694

1254394694



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kept in a room receiving natural light and maintained quiet. At around 13:00, we began Msum trials by measuring two birds in parał lei using the instruments and protocol described by Lewden et al. (2012) and Petit et al (2013). This was followed by a second trial on the remaining two birds, which began before 15:00. Measurements were carried out as follows. Birds were first weighed (± 0.1 g) and body temperaturę was measured with a thermocouple thermometer (Omega model HH-25KC, NIST-traceable, Omega, Montrćal, QC, Canada) using a copper-constantan thermocouple inserted into the cloacae approximately 10 mm deep. Then, birds were put in a stainless Steel metabolic chamber fitted with a perch and were exposed to dry, C02-free helox gas (21% oxygen, 79% helium) using an average flow ratę of 1109 ml.min'1. We recorded oxygen consumption of each bird using a sliding cold exposure protocol (Swanson et al.9 1996) with a decrease in ambient temperaturę of 3°C every 20 minutes, starting at 3°C in the fali, 0°C in winter and 6°C in summer. We ended the trials when birds became hypothermic which was easily identifiable in real time as a steady decline in oxygen consumption for several minutes. Body temperaturę was measured again immediately after taking the birds out of their chamber. We assumed a bird reached its Msum when body temperaturę after a trial was < 38°C (Cooper & Gessaman, 2005) (mean body temperaturę after Msum measurement = 34.1 ± 0.3°C). Data from individuals showing a body temperaturę above this threshold were discarded. Birds were weighed again after measurements and the average body mass was used for the Msum analysis. Birds were then brought back to their cage with food and water ad libitum until BMR measurement, starting at 19:00.

Each day, all four birds had their BMR measured simultaneously ovemight (from 19:00 to 06:00). Individuals were maintained at 30°C throughout the trial (within the thermoneutral zonę for this species, Rising & Hudson, 1974) and received a constant air flow of 470 ml.min'1. Birds were weighed before and after measurements and average mass was used in BMR analyses. Oxygen analyzers (FoxBox, Sabie Systems, Las Vegas, NV, USA) were adjusted each day using C02-free dry air. Mass flow valves (Sierra Instruments, Side-Trak® Model 840, Monterey, CA, USA) were calibrated for air and helox using a bubble-O-meter (Dublin, OH, USA). Metabolic ratę calculations were done with ExpeData software, v 1.2.6 (Sabie Systems, Las Vegas, NV, USA). Msum and BMR were calculated according to Lighton*s equation 10.1 (Lighton, 2008) and based respectively on the highest and lowest



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