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L929 cells with VSV induced a similar mcrease in apoptosis over time for ałl G mutants and for WT VSV. In contrast, the Mmj/r mutant showed a dramatic increase of activated caspases as early as 12 hours post-infection (P < 0.001) (Figurę 6A) correlating with the viability assay (Figurę 5). We also examined apoptosis induction in the B16 melanoma celi linę (Figurę 6B) and demonstrated that contrary to what we observed with L929 cells, some G mutants induce significantly morę programmed celi death compared to WT VSV or the Mmsir mutant (Gć compared to WT P < 0.05; Gsr, Gć and Gćr compared to Mm sir P < 0.01) also correlating with the viability assay (Figurę 5). We did not observe significant necrosis in any of the celi linę tested or virus used (data not shown).
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