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VI

CHAPITRE II

LOW-BONE-MASS PHENOTYPE OF DEFICIENT MICE FOR THE CLUSTER OF DIFFERENTLATION 36 (CD36)......................................................61

2.1    Avant-propos........................................................................................................62

2.2    Resume..................................................................................................................63

2.3    Abstract.................................................................................................................64

2.4    Introduction...........................................................................................................65

2.5    Materiał and methods...........................................................................................67

2.5.1    Animals......................................................................................................67

2.5.2    Plasma analysis..........................................................................................68

2.5.3    Documentation of bonę architecture..........................................................68

2.5.4    Bonę histochemistry..................................................................................69

2.5.5    Primary cultures of osteoblasts..................................................................70

2.5.6    Alcaline phosphatase activity....................................................................71

2.5.7    MTT activity..............................................................................................72

2.5.8    PCR amplification.....................................................................................72

2.5.9    Statistical analysis......................................................................................74

2.6    Results..................................................................................................................74

2.6.1    Body weight, bonę length and plasmatic markers.....................................74

2.6.2    Determination of bonę architecture...........................................................75

2.6.3    Plasma levels of bonę remodeling markers and bonę histochemistry.......76

2.6.4    Functions of osteoblasts from Cd36KO mice............................................76

2.7    Discussion.............................................................................................................78

2.8    Tables and figures ................................................................................................84

CHAPITRE III

SYSTEMIC AND CELLULAR ALTERATIONS IN CD36-DEFICIENT

MICE REDUCE SURVIVAL AND OSTEOGENIC POTENTIAL

OF MESENCHYMAL STROMAL CELLS .............................................................94

95

3.1 Avant-propos