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. Chromalograms obtained for 6 mm I.D. model columns under loadings of ca. 2 - 10”² g/g and 5 ■ 10“ ⁵ g/g ( —)- Mobile phasc: dichloromcthanc—mcthanol—water (91.2:8:0.8, v/v). Detector 254 nm) sensitivity: 0.01-1.28 a.u. LA, LB. LC = Lanatosides A, B and C, respectively. (a) Column — 2 cm-’, u — 2.5 mm/s; (b) column 4, V, = 1 cm³, u = 10 mm/s; (c) column 2, V, = 4 cm³.

The examples of chromatograms obtained during research on optimization of process scalę HPLC separation and obtaining of cardiac glycosides using NP.-HPLC and „model scalę" columns;

Important separation parameters should be chosen using „model scalę" column and afterthem recalculated for preparative or process scalę column;

The important parameters to should be optimized: dp, Lc, u (w), Ci, Vi, „sample" (feed) solvent, fraction collection points,