4613347723
Flow cytometry
Spleen and tumors were recovered from mice and dissociated to achieve single-cell suspensions using a nylon 100 pm celi strainer (BD Falcon). Cells were washed, resuspended in phosphate-buffered salinę containing 1% bovine serum albumin and 0.1% sodium azide (FACS buffer), and incubated with directly conjugated primary antibodies for 30 minutes at
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