4613347779
Celi lines
Murine BI6 and B16gp33 melanoma celłs were obtained from Dr. A. Ochsenbein (Bern, Switzerland). The B16gp33 celi (H2-Db) were derived from B16.F10 cells transfected with a DNA minigene encoding the immunodominant CD8+ T celi epitope of the lymphocytic choriomeningitis virus (glycoprotein aa 33-41) (Prevost-Blondel et al., 1998). Cells were grown in Dulbecco's modified Eagle's medium (Life Technologies, Rockville, MD) supplemented with 10% fetal bovine serum (FBS) and 200 pg/ml of G418 to select for retention of the gp33 minigene.
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