869568748

SUB-DEPARTMENT OF VETERINARY MICROBIOLOGY

Akademicka 12. Lublin

Hcad: Prof. extraordin. Dr. habil. Grażyna Ziółkowska Dr. habil. Barbara Majer-Dziedzic

RESEARCH STUDIES (SUMMARIES)

Irena Choina. Edyta Grzelak, Wioletta Bąk, Karol Pilorz,

Barbara Majer-Dziedzic

DETERMINATION OF ENROFLOXACIN AND CIPROFLOXACIN IN MILK BY DIRECT BIOAUTOGRAPHY DETECTION

Fluoroąuinolones are widely used in the treatment of both human and veteri-nary diseases. Ciprofloxacin, one of the most popular human antibiotics, is a main metabolite of enrofloxacin. In veterinary medicine it is mainly used to cure mastitis and cows. Hence, both of tliis dmgs are potential residues in the milk of treated cows. Analytical methods should be sensitive and selective enough to distinguish between these antibiotics at their maximum residua level (MRL), wliich is lOOpg/kg for the sum of these drugs. HPLC, most freąuently used in antibiotic analysis, can often be replaced advantegously by HPTLC/TLC-DB enables the separation of antibiotics, and at the same time, detection of their antibacterial properties. The developer piąte in dipped directly in bacterial sus-pension. After incubation and visualization, an inhibition of bacterial growth around the antibiotic zones can be observed. The method is simple, veiy sensi-tive, accurate and precise. Its great advantage over HPLC is not only the analysis of many samples at the same time but also the additional infonnation about their antibacterial properties. The detection of any further antibacterial compounds, which are not known (e.g. break down products) or not in the fokus of the regu-lar target analysis, is treasured as well.

Publication: Planar Chromatography in Practice. 106, 2-4, 2011, fig. 2. In English, summary in English.

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