How can I minimize nonspecific adsorption on a surface plasmon resonance (SPR) sensor chip?

How can I minimize nonspecific adsorption on a surface plasmon resonance (SPR) sensor chip? May 25, 2013 · Flag

• Fuxing Gu · 19.93 · 58.05 · University of Shanghai for Science and Technology

Tell us what is your "nonspecific adsorption"? 19 hours ago

Teodor Aastrup · 26.47 · 80.8 · Active Biotech

If you are running crude samples, like cell lysate or hybridomas or sera samples or have general non-specific challenges, I recommend that you use the LNB-chip and the A200 biosensor from Attana. Attana has developed the qcm-technology in order to meat this challanges. 18 hours ago

• Ugur Kayran · 0.49

You can try to wash fast (high flow rate) 17 hours ago

• Mauro Acchione · 17.94 · 53.91 · University of Washington Seattle

More details on your specific system of interest that you are studying will provide better answers. But without knowing the details, I can say that for proteins, BSA (up to 3%) in the assay/running buffer is often used in systems from different companies to lower NSB. Small amounts of non-ionic detergent (Tween, Triton, etc.) also can be of help if they are compatible with your system. Anything that has been used to reduce NSB in other bi-molecular assays like ELISA could work in your system. If you are having issues with NSB binding with small hydrophobic molecules then increasing or including cosolvent (DMSO/MeOH) can be of help, so long as you can correct for the signal response from these cosolvents. There are other things to explore with particular chips and immobilization strategies but I would need more details about the system.

1 / 0 · 16 hours ago

• Johana Kuncova-Kallio · 3.66 · 1.46 · BioNavis

You can either work further on your surface, such as use blocking molecules, N,N-bis (2-hydroxyethyl)-α-lipoamide (Vikholm-Lundin et al. Surface Science 603 (2009) 620–624) or Ova (Susanna Sonny, Adama M. Sesay, Vesa Virtanen, Proc. SPIE 7376, 737605 (2010)) for instance.
The other option is to use Selectively Amplified Surface Plasmon Resonance, which is a kind of a color-dye used with Multi-Parametric Surface Plasmon Resonance instruments and gives more than 120x improvement in Signal-to-Noise ratio.

1 / 0 · 15 hours ago

• Rudolf Robelek · 23.44 · 65.84 · Universität Regensburg

To generally avoid nonspecific binding on Au surfaces try to flush the surface with BSA before performing your final (interaction) measurement step.

In case you build up your own sensor surface architecture starting from pure Au surfaces, try to flush the surface with a short chained thiol compound or cystein, resp.. This will close open Au spots and help to avoid unwanted contacts between analyte and sensor surface. 5 hours ago

• Eva Muñoz · 24.73 · 74.01 · Universidad de Santiago de Compostela

I completelly agree with Mauro Acchione´s comments. Besides, I would say that you need to to identify the source and nature of the non-specific binding. It is non specific binding to the ligand or to the chip surface? what type of interaction is it? answers to these questions will allow you to improve your experimental set up e. g. by changing the type sensor surface or including additives to your rurring buffer...