Use of DNA DNA profiling DNA typing DNA fingerprinting Discovered by Professor Sir Alec Jeffreys Sir Alec Jeffreys 1 Use of DNA Restriction Fragment Length Polymorphism (RFLP) Needed large amounts of sample Use non-coding region of DNA Why? 2 Use of DNA Most of what makes us human beings is common to us all Few minor differences 3 Use of DNA Coding region Not highly individualised Junk DNA High variability 4 RFLP Can use more than one DNA probe Bill Clinton 7 probes used More probes Higher probability 5 Gel Electrophoresis Analogous to TLC Capillary gel electrophoresis Analogous to HPLC 6