4 2 Techniques used in DNA Profiling v1

Use of DNA
DNA profiling
DNA typing
DNA fingerprinting
Discovered by Professor
Sir Alec Jeffreys
Sir Alec Jeffreys
1
Use of DNA
Restriction Fragment Length Polymorphism (RFLP)
Needed large amounts of sample
Use non-coding region of DNA
Why?
2
Use of DNA
Most of what makes us human
beings is common to us all
Few minor differences
3
Use of DNA
Coding region
Not highly individualised
Junk DNA
High variability
4
RFLP
Can use more than one DNA probe
Bill Clinton 7 probes used
More probes Higher probability
5
Gel Electrophoresis
Analogous to TLC
Capillary gel electrophoresis
Analogous to HPLC
6

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