Vitroids"! Certified Reference Microorganisms
Sigma-Aldrich® Worldwide Offices
Cat. No. Certified Reference Material Origin Strain No. Approx. Mean CFU per Disc Acceditation
Certified Reference
RQC22003 Acinetobacter baumanni ATCC® 19606 80 P
RQC22005 Acinetobacter baumanni ATCC® 19606 200 P
Argentina Germany Malaysia Spain
RQC22007 Acinetobacter baumanni ATCC® 19606 1000 P
Free Tel: [phone] Free Tel: [phone] Free Tel: [phone]
Tel: [phone]
RQC15003 Aspergillus brasiliensis ATCC® 16404"! 80 P
Free Fax: [phone]
Tel: [phone] Free Fax: [phone]
Fax: [phone]
RQC16003 Bacillus subtilis ATCC® 6633"! 80 P
Microorganisms
Tel: [phone]
Fax: [phone]
RQC02258 Bacillus subtilis ATCC® 6633"! 10'000 P Tel: [phone]
Mexico
Fax: [phone]
RQC14003 Candida albicans ATCC® 10231"! 80 P
Fax: [phone]
Australia
Free Tel: [phone]
RQC14004 Candida albicans ATCC® 10231"! 100 P
Hungary
Free Tel: [phone]
Sweden
Free Fax: [phone]
RQC14007 Candida albicans ATCC® 10231"! 1'000 P
Tel: [phone] The Simple Way to Ensure Accurate Results, Every Time
Free Fax: [phone]
Tel: [phone]
RQC14008 Candida albicans ATCC® 10231"! 10'000 P
Tel: [phone]
Fax: [phone]
Tel: [phone]
RQC02105 Citrobacter freundii ATCC® 8090"! 200 P Fax: [phone]
Fax: [phone]
RQC02108 Citrobacter freundii ATCC® 8090"! 10'000 P Fax: [phone] India
Switzerland
RQC19003 Clostridium sporogenes ATCC® 19404"! 80 P Telephone The Netherlands
Austria
Free Tel: [phone]
Vitroids"! and LENTICULE® Discs
RQC01652 Enterobacter aerogenes ATCC® 13048"! 50 P
Bangalore: ([phone] Tel: [phone]
Tel: [phone]
Free Fax: [phone]
RQC01654 Enterobacter aerogenes ATCC® 13048"! 100 P
New Delhi: ([phone]
Fax: [phone]
Fax: [phone]
" Defined CFU & low standard deviation (ISO/IEC 17025)
RQC01655 Enterobacter aerogenes ATCC® 13048"! 200 P Tel: [phone]
Mumbai: ([phone]
RQC01657 Enterobacter aerogenes ATCC® 13048"! 1'000 P
Belgium New Zealand Fax: [phone]
" Economic, convenient and easy to use
Pune: ([phone]
RQC01658 Enterobacter aerogenes ATCC® 13048"! 10'000 P
Tel: [phone] Free Tel: [phone]
Hyderabad: ([phone]
Thailand
" Long shelf life
RQC21102 Enterobacter cloacae ATCC® 35030"! 50 P
Fax: [phone]
Kolkata: ([phone] Free Fax: [phone]
Tel: [phone]
RQC21105 Enterobacter cloacae ATCC® 35030"! 200 P
Brazil Tel: [phone]
RQC01772 Enterococcus faecalis ATCC® 19433"! 50 P Fax Fax: [phone]
RQC01774 Enterococcus faecalis ATCC® 19433"! 100 P Free Tel: [phone] Fax: [phone]
Bangalore: ([phone]
United Kingdom
RQC01775 Enterococcus faecalis ATCC® 19433"! 200 P
Tel: [phone]
New Delhi: ([phone]
Norway Free Tel: [phone]
RQC01776 Enterococcus faecalis ATCC® 19433"! 500 P
Fax: [phone] Mumbai: ([phone]
Operated by Public Health England Operated by Public Health England
Tel: [phone] Free Fax: [phone]
RQC01777 Enterococcus faecalis ATCC® 19433"! 1'000 P
Pune: ([phone]
Canada
RQC20003 Enterococcus faecium ATCC® 19434"! 80 P Fax: [phone] Tel: [phone]
Hyderabad: ([phone]
RQC20005 Enterococcus faecium ATCC® 19434"! 200 P Free Tel: [phone]
Fax: [phone]
Poland
Kolkata: ([phone]
RQC20007 Enterococcus faecium ATCC® 19434"! 1000 P
Free Fax: [phone]
United States
Tel: [phone]
RQC01702 Escherichia coli ATCC® 11775"! 50 P
Ireland
Tel: [phone]
Toll-Free: 800 325 3010
RQC01705 Escherichia coli ATCC® 11775"! 200 P Fax: [phone]
Free Tel: [phone]
Fax: [phone]
RQC01707 Escherichia coli ATCC® 11775"! 1'000 P Toll-Free Fax: [phone]
Free Fax: [phone]
Portugal
Chile
RQC01708 Escherichia coli ATCC® 11775"! 10'000 P
Tel: [phone]
Tel: [phone]
Free Tel: [phone]
RQC02704 Escherichia coli ATCC® 25922"! 100 P Tel: [phone]
Fax: [phone]
Fax: + 353 (0) 402 20375
RQC02708 Escherichia coli ATCC® 25922"! 10'000 P Free Fax: [phone]
Fax: [phone]
Vietnam
RQC11003 Escherichia coli ATCC® 8739"! 80 P Israel
Tel: [phone]
People s Republic of China
RQC02504 Heterotrophic Organisms   100 P Free Tel: [phone] Tel: [phone]
Fax: [phone]
Free Tel: [phone]
RQC02601 Klebsiella pneumoniae ATCC® 13883"! 30 P
Tel: [phone] Fax: [phone]
Tel: [phone]
RQC02607 Klebsiella pneumoniae ATCC® 13883"! 1000 P
Russia
Fax: [phone]
Internet
RQC03105 Klebsiella pneumoniae ATCC® 33495"! 200 P Fax: [phone]
Free Tel: [phone]
Italy
sigma-aldrich.com
RQC03107 Klebsiella pneumoniae ATCC® 33495"! 1000 P
Czech Republic Tel: [phone]
Free Tel: [phone]
RQC01901 Listeria monocytogenes ATCC® 19115"! 30 P
Tel: [phone]
Fax: [phone]
Tel: [phone]
RQC03207 Proteus hauseri ATCC® 13315"! 1000 P
Fax: [phone]
RQC03407 Proteus mirabilis ATCC® 25933"! 1000 P Fax: [phone]
Singapore
RQC01855 Pseudomonas aeruginosa ATCC® 10145"! 200 P
Denmark
Japan Tel: [phone]
RQC03302 Pseudomonas aeruginosa ATCC® 27853"! 50 P
Tel: [phone]
Tel: [phone]
Fax: [phone]
RQC03304 Pseudomonas aeruginosa ATCC® 27853"! 100 P
Fax: [phone]
Fax: [phone]
RQC03307 Pseudomonas aeruginosa ATCC® 27853"! 1'000 P
Slovakia
RQC02201 Pseudomonas aeruginosa ATCC® 9027"! 30 P Finland
Korea
Tel: [phone]
RQC12002 Pseudomonas aeruginosa ATCC® 9027"! 50 P
Tel: [phone]
Free Tel: [phone]
Fax: [phone]
RQC12003 Pseudomonas aeruginosa ATCC® 9027"! 80 P
Fax: [phone]
Free Fax: [phone]
RQC02204 Pseudomonas aeruginosa ATCC® 9027"! 100 P
South Africa
Tel: [phone]
France
RQC12005 Pseudomonas aeruginosa ATCC® 9027"! 200 P
Fax: [phone] Free Tel: [phone]
Free Tel: [phone]
RQC12007 Pseudomonas aeruginosa ATCC® 9027"! 1'000 P
Free Fax: [phone]
RQC03006 Salmonella enterica subsp. enterica serovar Abaetetuba ATCC® 35640"! 500 P Free Fax: [phone] Luxembourg
RQC03007 Salmonella enterica subsp. enterica serovar Abaetetuba ATCC® 35640"! 1'000 P Tel: [phone]
Tel: [phone] Tel: [phone]
RQC17002 Salmonella enterica subsp. Enterica serovar Typhimurium ATCC® 14028"! 50 P
Fax: [phone]
Fax: [phone] Fax: [phone]
RQC17003 Salmonella enterica subsp. Enterica serovar Typhimurium ATCC® 14028"! 80 P
RQC13002 Staphylococcus aureus susp. Aureus ATCC® 6538"! 50 P
RQC13003 Staphylococcus aureus susp. Aureus ATCC® 6538"! 80 P
RQC13004 Staphylococcus aureus susp. Aureus ATCC® 6538"! 100 P
Order/Customer Service: sigma-aldrich.com/order World Headquarters
Enabling Science to
RQC13005 Staphylococcus aureus susp. Aureus ATCC® 6538"! 200 P
3050 Spruce St.
Technical Service: sigma-aldrich.com/techservice
Improve the Quality of Life
RQC13007 Staphylococcus aureus susp. Aureus ATCC® 6538"! 1'000 P St. Louis, MO 63103
Development/Custom Manufacturing Inquiries [e-mail]
[phone]
RQC0001 Vitroids"! Blank   0 P
Safety-related Information: sigma-aldrich.com/safetycenter
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©2015 Sigma-Aldrich Co. LLC. All rights reserved. SAFC and SIGMA-ALDRICH are trademarks of Sigma-Aldrich Co. LLC, registered in the US and other countries. Vitroids is a trademark of Sigma-Aldrich Co. LLC.
ATCC is a registered trademark of American Type Culture Collection. ATCC Licensed Derivative® Emblem, the ATCC Licensed Derivative® word mark, and the ATCC catalog marks are trademarks of ATCC.
ATCC Licensed Derivative® Emblem, the ATCC Licensed Derivative® word mark, and the ATCC catalog marks are trademarks of ATCC.
Sigma-Aldrich is licensed to use these trademarks and to sell products derived from ATCC® cultures. LENTICULE® discs are manufactured by Sigma-Aldrich under license from Public Health England. LENTICULE® RTO
Sigma-Aldrich is licensed to use these trademarks and to sell products derived from ATCC® cultures.
discs are manufactured by Sigma-Aldrich under licence from Public Health England. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. 50702
Please see product information on the Sigma-Aldrich website at www.sigmaaldrich.com and/or on the reverse side of the invoice or packing slip. 1085
Vitroids"! are prepared from a traceable culture obtained freeze-dried
Save Time and Costs LENTICULE® discs Certified Reference Microorganisms
from the American Type Culture Collection (ATCC®) and produced
The Simple Way to Ensure Accurate Results,
Using Vitroids"! and LENTICULE® discs is time saving because it Cat. No. Reference Material Origin Strain No. CFU Range Approx. CFU per disc Accreditation
according a Sigma-Aldrich patented technology.
removes the need for preparing stock cultures. The organisms need RMF02275L Aspergillus brasiliensis (formerly Aspergillus niger) NCPF® 2275 Low Level 30 120 
no recovery time and no pre-enrichment step. In addition the product
Every Time CRM07464L Bacillus cereus NCTC® 7464 Low Level 30 120 P
A New Partnership
concentrations are designed in a range where no or only minimal
CRM07464M Bacillus cereus NCTC® 7464 Medium Level 500 5 × 104 P
Certified Reference Microorganisms
dilutions are needed. The discs readily dissolve in liquid media and on
Public Health England's NCTC® is a national BRC that preserves,
RM11322Q Campylobacter jejuni NCTC® 11322 Low Level 102 
agar plates resulting in easy handling and a very economical solution.
maintains and regularly updates a specific collection of bacterial
RMF03255L Candida albicans NCPF® 3255 Low Level 30 120 
strains, ensuring there are no changes to physical characteristics,
RMF03255H Candida albicans NCPF® 3255 High Level 2 × 104 
What is New Compared to Existing Reference
such as morphology and nutritional requirements, the genome and
RM09750L Citrobacter freundii NCTC® 9750 Low Level 30 120 
Strain Products? the proteome. It is one of several BRCs that provide authenticated
The discs contain pure cultures of bacteria, yeasts or moulds in a
Why use CRMs in Microbiology?
CRM00506L Clostridium bifermentans NCTC® 506 Low Level 30 120 P
biological reference and control strains. In addition, the PHE also
solid water-soluble matrix. Comprehensive certificates of analysis
Utilization of new technology has allowed us to make major
In food, water and environmental microbiology, laboratory results
CRM13170L Clostridium perfringens NCTC® 13170 Low Level 30 120 P
developed the LENTICULE disc that enables a laboratory to have an
specify the mean number of colony forming units (CFU) per disc,
improvements in the field of Microbiological Reference Materials.
are an important part of a wider process that helps to confirm that
on demand source of a control for quantitative microbiology. CRM13170M Clostridium perfringens NCTC® 13170 Medium Level 500 5 × 104 P
details about the method used to determine the product data and
The main areas of development are stability, temperature resistance,
samples are of an acceptable microbiological quality, are safe and
the number of subcultures from the original strains, provided under CRM11467L Cronobacter sakazakii NCTC® 11467 Low Level 30 120 P
adjusting the narrow defined CFU range, rehydration time and
With increased worldwide demand for the accreditation of testing
comply with relevant legislation or guidelines. Quality control is an
licence by NCTC® and ATCC®.
CRM10006L Enterobacter aerogenes NCTC® 10006 Low Level 30 120 P
better within batch reproducibility. In addition, each disc is certified
labs, as well as the development of fast, automated methods in
essential element of a laboratory s quality assurance system and
according to ISO Guide 34 and ISO/IEC 17025. CRM10006M Enterobacter aerogenes NCTC® 10006 Medium Level 500 5 × 104 P
microbiology, the use of Microbial Certified Reference Materials has
characterised authenticated reference materials are necessary for Single-use controls manufactured directly from cultures provided
increased substantially. With this in mind, it is an appropriate time to CRM00775L Enterococcus faecalis NCTC® 775 Low Level 30 120 P
effective quality control. by recognised Biological Resource Centres (BRCs) such as NCTC®
Preparation
entrust the manufacture and distribution of these products to an ISO
and ATCC® mean that laboratories can be confident about the CRM00775M Enterococcus faecalis NCTC® 775 Medium Level 500 5 × 104 P
The same is true of
Most solid and liquid medium or accredited manufacturing company such as Sigma-Aldrich, enabling
authenticity of their strains and the suitability of their quality control
CRM00775H Enterococcus faecalis NCTC® 775 High Level >105 P
microbiological testing; the
rehydration buffer can be used. Discs can the PHE organization to focus on research and development for new
materials, factors that are of increasing importance as laboratories
CRM09001L Escherichia coli NCTC® 9001 Low Level 30 120 P
one factor that is repeatedly
be rehydrated in as little as 100 µL buffer, products to add to this portfolio. This R&D is further enhanced by
become more automated and new technologies emerge and are
CRM09001M Escherichia coli NCTC® 9001 Medium Level 500 5 × 104 P
overlooked is careful sourcing of
or in larger volumes, e.g. 100 mL medium. an exciting project by PHE, in collaboration with the Wellcome Trust
rapidly adopted in routine microbiology settings.
biological resources such as the CRM09001H Escherichia coli NCTC® 9001 High Level >105 P
It is also possible to add the disc to a Sanger Institute (WTSI), to provide whole genome sequences using
quality control strains. Incorrect
CRM13216L Escherichia coli NCTC® 13216 Low Level 30 120 P
cooled molten medium used for pour long-read technology for 3000 bacteria of clinical importance.
What are Vitroids"! and LENTICULE® discs?
quality control materials may
CRM12900L Escherichia coli O157 (NT) NCTC® 12900 Low Level 30-120 P
plate techniques. The rehydration process
Vitroids"! and LENTICULE® discs contain viable microorganisms in a An integral part of this new partnership is Sigma-Aldrich s creation
indicate that test results are
takes approximately 10 minutes. On solid CRM08167L Klebsiella oxytoca NCTC® 8167 Low Level 30 120 P
certified quantity (generally accredited according ISO/IEC 17025), of a new, dedicated manufacturing facility in Buchs, Switzerland
acceptable when, in fact, there is a
media, the disc forms a droplet that can
CRM11368M Legionella bozemanii NCTC® 11368 Medium Level 500 5 × 104 P
produced under reproducible conditions compliant with ISO Guide to provide the growth and development of Certified Reference
problem with the samples being
be spread with a sterile loop. In liquid
CRM11371M Legionella micdadei NCTC® 11371 Medium Level 500 5 × 104 P
34:2009 using authenticated strains from NCTC®, NCPF® and ATCC®. Microorganisms, both for now and for the future. It will enable more
tested. Alternatively, control results
media, the disc dissolves very quickly.
CRM12821L Legionella pneumophila NCTC® 12821 Low Level 30 120 P
Consisting of pure cultures of bacteria or fungi in a solid water scientists worldwide to easily access the NCTC®/NCPF® CRMs through
may indicate that a test is not
soluble matrix, they are stable for at least one year and are in a viable the global supply chain of Sigma-Aldrich.
performing correctly, instigating CRM12821M Legionella pneumophila NCTC® 12821 Medium Level 500 5 × 104 P
Packaging
state with a shelf life of 1-3 years. The within batch variation for every
unnecessary investigations and CRM11288L Listeria innocua NCTC® 11288 Low Level 30 120 P
The discs are packed individually in
product is very low (in some cases less than 4% standard deviation). The Benefit of Combined Experience
repeat testing.
CRM11994L Listeria monocytogenes NCTC® 11994 Low Level 30 120 P
vials. The vials have a special screw-cap
Each batch is provided with a comprehensive certificate of analysis
In recent years Sigma-Aldrich, in partnership with Zurich University
CRM11994M Listeria monocytogenes NCTC® 11994 Medium Level 500 5 × 104 P
Ready-to-use microbiological controls minimise the need for
with seal and contain a desiccant at
that specifies the mean number of colony forming units (CFU), an
of Applied Sciences (ZHAW), has gained a great deal of experience
maintaining control strains in the test laboratory and guarantee that RMBLANK0 Negative Control (No bacterial growth expected) (0 CFU)     
the bottom or in the cap. The vials are
expanded uncertainty about the mean, details about the method
developing an excellent range of Certified Reference Microorganisms
an authenticated control culture is used for every quality control
packed in a mylar bag with a zip. CRM10662L Pseudomonas aeruginosa NCTC® 10662 Low Level 30 120 P
used to determine the product data and the number of passages
(CRM) called Vitroids"!. The process was standardized and CRM
test. Such control materials must be fit-for-purpose, bearing in
CRM10662M Pseudomonas aeruginosa NCTC® 10662 Medium Level 500 5 × 104 P
(subcultures) from the original strain.
quality improved to a level superior to today s standard. Since 2008,
mind that for food, water and environmental samples the ability to
Version 1 Version 2 CRM09528L Raoultella planticola (formerly Klebsiella aerogenes) NCTC® 9528 Low Level 30 120 P
knowledge has been acquired to extend production according to
accurately enumerate bacteria, yeasts and moulds and reliably detect
Applications (only used for Vitroids"!) (used for Vitroids"! & LENTICULE® discs)
CRM09528M Raoultella planticola (formerly Klebsiella aerogenes) NCTC® 9528 Medium Level 500 5 × 104 P
ISO guide 34 and certification according to ISO/IEC 17025 to the
relatively low numbers of pathogenic organisms is essential. It is also
RMF03191L Saccharomyces cerevisiae NCPF® 3191 Low Level 30 120 
QC to assure the quality of test results (water, food, beverage,
" field of microbiology. With two manufacturing and development
important that controls can be applied to the wide range of different Desiccant
Screw
environmental etc) sites, one in Laramie, Wyoming U.S.A. and the new one in Buchs, RMF03191M Saccharomyces cerevisiae NCPF® 3191 Medium Level 500 5 × 104 
food and water matrices that are often tested in a single laboratory.
Cap
Switzerland, fufills this growing demand for microbial CRMs.
CRM06676L Salmonella Enteritidis NCTC® 6676 Low Level 30 120 P
Performance testing of media acc. ISO 11133
"
The application of a unique
Combining the know-how and capabilities of Sigma-Aldrich and PHE
CRM07832L Salmonella Nottingham NCTC® 7832 Low Level 30 120 P
Validation of new methods
"
preservation technology
will benefit microbiologists all over the world.
CRM12023L Salmonella Typhimurium NCTC® 12023 Low Level 30 120 P
involving controlled-drying
Materials for proficiency testing or ring trials
"
Filter Plug
CRM06571L Staphylococcus aureus NCTC® 6571 Low Level 30 120 P
of authenticated cultures
Method development
"
CRM06571M Staphylococcus aureus NCTC® 6571 Medium Level 500 5 × 104 P
of internationally accepted CRM Disc
Desiccant
Staff training
" CRM11047L Staphylococcus epidermidis NCTC® 11047 Low Level 30 120 P
microbiology control strains
has resulted in the production RM11218Q Vibrio furnissi NCTC® 11218 Low Level 30-120 
Starter cultures
"
of single-use discs containing
RM10903Q Vibrio parahaemolyticus NCTC® 10903 Low Level 30 120 
microorganisms, designed for use
RM11176L Yersinia enterocolitica NCTC® 11176 Low Level 30 120 
Stability
in food, water and environmental
testing laboratories. These Certified Reference Microorganisms in this unique format are very
Strains
Get a FREE sample! of Vitroids"! & LENTICULE® discs*
stable and in most cases will remain so for many years at -20 °C.
quality control materials, LENTICULE® discs (developed by Public
The numbers of CFUs do not change, the organisms need no LENTICULE® discs are prepared from a traceable culture obtained For more information and a list of Certified Reference
Health England, PHE) and Vitroids (developed by RTC), are now
available from Sigma Aldrich, and are manufactured under recovery time and have no lag phase. Even a short period at freeze-dried from the National Collection of Type Cultures (NCTC®) or Microorganisms, visit sigma-aldrich.com/mibi-crm
Operated by Public Health England Operated by Public Health England
ambient temperature, such as during shipment, is not an issue National Collection of Pathogenic Fungi (NCPF®) and are manufactured
conditions compliant with ISO Guide 34:2009 (General *Mention promo code SFV when placing your order.
Limit one package per product and per customer; no shipping fee.
LENTICULE® discs are manufactured by Sigma-Aldrich® under licence from Public Health England.
requirements for the competence of reference material producers). for product stability. by Sigma-Aldrich® under license and control from Public Health England. The Sigma-Aldrich facility in Buchs, Switzerland.
sigma-aldrich.com/mibi-crm