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Name and Intended Use__—--

RockChek* Avtan Pneumovims łs DBOCs enzyme immunoassay for the detection of antibody to avian pneumavirus (APV) in ctiicken and turkey serum

General Information ____——

Amn Pneumodrus (APV) is a pathogen of chickens and turkeys that can produce respiratory signs and reproductwe failures as a primary paihogen, or as part of a multiagent syndrome known as Swollen Head Syndrome (SUS). Vinis isolation of APV is a difficult task, so diagnosis through seroiogy is one of the most important toofs for cfcease control. This test is intended for quantitative monitoring chicken and turkey ftocks that have been wcdnated with !ive and/or inactwated products containing A. B or C antigens, as wełl as naive (SPF) flocks for the detection of infected btrds with any A. B or C serotypeof APV.

Descriptions and Princlples_____________

This assay is designed to measure the rełatwe levei of antibody to APV in chicken and turkey serum. Viral antigen is coated on 96-weti ptates. Upon incubaOon of the test sampte in the coated wełl, antibody specific to APV forms a comptex witti the coated viral antigens. After washing away unbound materiał from the wells, a conjugate is added that binds to any attached chicken and turkey antibody in the wełfe. Unbound conjugate is washed away and enzyme substrate is added. Subsequent color devełopment is directfy rełated to the amount of antibody to APV present in the test sampte.

Reagent __ -

Stora al reagents at 2°- 8°C

		Volume
1.	AfYcoated ptates	5 piates
2.	AFV PosSwe control	1.9 mi
1	Negatwe control	1.9 ml
4.	AnthQickerVAn6-Turiey Horserarfish Peraodase conjugate	50 ml
5.	Sampte (Buent buffer	235 ml
8.	TMB substrate	60 ml
C.	StapsoUton	60 ml

Materials Required (Not Pnnnded)    ‘. '..1

•    Prcasjon pęettes and muftpie dekvery f*petting device witti dtsposabie p«pette tips

•    96-weX piąto raader

•    TtibestorrButingsamptes

•    Dsafeiordeionśzedwater

•    Derce tor the defrery and aspirabon of wash solubon

Precaiftions and Wamings for Users___

•    Hande al biotogical materiał as potentialy infedtous.

•    Do not pipette by moutfi.

•    Do rwteatetonfcorsmokenherespedmensor kit reagents are being handied.

•    The substrate solution is anrftating to eyes, respiratory system and skin. Avoid contact with skin and eyes.

•    Do not exposeTMBsoliflłon to strong&ghtorsiyoxkkzingagenrs. Handle TMBsotution with deaigfassorpiasdcware.

•    Storę al reagents at 2°-8°C- Bring the reagents to room temperaturo (18°-25°Q prtor to use, and return to 2°-8°C fbflowtog use.

•    Al wastes shoiśd be property decontaninated prior to deposal.

•    (^reshoildbetatentopremrtcontarninafaiofklcomponents.

•    Do not use components past toeir ei^iirabon dates and do not mix components from cifferent seriab.

•    Opomd resurs w* be obtatoed by sttct atfierence to tfus protocoi. Carefulpipetting, timing and washing tfiroughout this procedura are rtecessary to maintain precson and acoiacy.

•    The tmcorOotsmust be used tor each test senes.

2 RockChek APV Ab