■ Sputum culture after concentration
or digestion with KOH_
(destroys other undesired organisms)
■ Collect several specimens.
■ Collect sputum early in the morning (increases bacterial concentration)
■ Acid-fast staining (Ziehl-Neilson or Krnyourr)
■ Fluorescent microscopy techniques
■ Culture of the organisms on specializęd media (L-J and Middlebrook agars)
■ PPD skin testing (DTH>