img038 (10)

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UTR

8

L

UTR*

mRNA

(1451 bp)

protein

(369 aa: 64 kDa)

signal

peptide

FN3 TM Hotloops

IGH gene rearrangement

V D J    Cu

Vh — Dh-Jh joining

HHl--■■■■

k    ____ _/

I transcription

precursor IGH mRNA

J    RNA splicing

maturę IGH mRNA

CD132 gene (Xq13.1; 4.1 kb)

1    2    3    4

-Eh

UTR

5

6

7

8

UTR

isA

-EJ I

79stopcodon

mRNA (1451 bp)

protein (369 aa, 64 kDa)

signal    FN3 TM Hotloops

peptide

IL-9R and IL-14R

Junctional region diversity of IGH VH-JH rearrangement

VH3-21 DH3-3 JH4-1    Cfl

Vh3-21 (germline) insertion    Dh3-3 (germline)    insertion Jh4-1 (germline)

[GTATTACGATTTTTGGAGTGGTTATTATACC

TGTATTACTGT

TGTATTACTGTGCG

T GTATTACT GTGC

TGTATTACTGTGCGAG

TGTATTACTG

TGTATTACTGTGCG

TGTATTACTGTGCGA

TGTATTACT

TGTATTACTGTGCG

TGTATTACTGTGC

T GTATTACT GT GCGAG A

TGTATTACTGTG

TGTATTA

TGTATTACTGTGC

TGTATTACTGTGCGA

-	TGACTACT
CGATCG	CTTTGACTACT
GGT	ACTACTTTGACTACT
CGTAGCGTA	TTTGACTACT
CGT AG	ACTTTGACTACT
GGCTAAGG	TGACTACT
CGGAGC	TACTTTGACTACT
GOTTC	ACTACTTTGACTACT
CGATCGA	CTTTGACTACT
CC	ACTTTGACTACT
GTCG	GACTACT
CCGTAG	CTACTTT GACTACT
C	ACT
GTACG	TTTGACTACT
GGCA	ACTTTGACTACT

AGGC    CGATTTTT GG AGT GGTTATTATA

TATCCGGA    TTACGATTTTT GGAGT GGTTATTATAC

CCGOACTO    TTTT GGAGT GGTTATTATAC C

ctgagtc    TATTACGATTTTT GGAGT GGTTAT

ACATCGA    CGATTTTT    GGAGT GGTTATTATA

CGT    TACGATTTTTGGAGTGGTTATTAT

CCC.G    TTACGATTTTTGGAGTGGTTATTATACC

gatg    GATTTTT    GGAGT GGT

rTCA ATTACGATTTTTGGAGTGGTTATTATA TTTGGAGTGGTTATTATA

; ).%(-,    ATTACGATTTTTGGAGTGGTTATTATACC

OIOCAC • TATTACGATTTTT GGAGT G ccgga    CGATTTTTGGAGTGGTTATTATA

ACOC ATTACGATTTTT GGAGTGG CGTA GTATTACGATTTTTGGAGTGGTTATTATACC