117828244

(b)

Figurę 3

De os It ogram (a), vldeoscan <b) obtained from the essential oil of S. hlans, and mass spectra of separatad chromatographic bands (1H⁵)-

light from a deuterium lamp at 340 nm. The dimensions of the rcctangular light beam were 2.0 mm * 0.1 mm. The dcnsi-tograms obtained were primarily assessed for providing a fin-gerprint response. Chromatograms were also scanned at 254 nm by use of a Chromimagc flatbed scanner (AR2i, Le Plessis Robinson, France), and the images of the chromatograms were saved. Fach TLC analysis was performed in triplicatc.

2.4 Mass Spectrometrlc Analysis ol Chromatographic Bands

After densitometry to locatc the separated chromatographic bands, bands were selected for mass spectrometric analysis. A CAMAG (Muttenz, Switzerland) TLC-MS Interface was used for d irect clution of a given band from the piąte and its On

Low-Temperature TLC-MS of Essential Ols

line introduction into a Varian (Harbor City, CA, USA) 500-MS mass spcctrometer. In our study, clution was performed at ambi-ent temperaturę by use of methanol (flow ratę 0.2 mL min ^;). Samples were analyzed in ESI modę (fuli ES1-MS scan, positive ionization, spray chamber temperaturę 45°C, drying gas temperaturę 150°C, dry ing gas pressure 25 psi, capillary potential 70 V, ncedle voltagc 5 kV). Varian MS Workstation v. 6.9.1 software was used for data acquisition and proccssing.

3 Results and Discussion

In prcvious papers [10, 11], we presented results from GC-MS analysis of the essential oils from different sagę species. ldenti-

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Joumal of P1an3r Chromatography 23(2010)4