3106269184

3106269184



the active hydrophobic State optima! at pH 6 that initiates fusion and the post-fusion State (Roche et al., 2006; Roche et al., 2007). Interactions between G and M proteins have also been shown to increase budding efficiency (Swinteck and Lyles, 2008). Apart from roles in fusion and particie assembly, G has recently been shown to also participate in cytotoxicity (Hoffmann et al., 2010) and in certain conditions, oncolysis (Wollmann et al., 2010).

VSV carrying mutant M proteins have been extensively studied (Lichty et al., 2004). When mutated at methionine 51, the M protein fails to błock host gene expression thereby allowing the celi to secrete type-I interferons (IFN) (Garcia-Sastre and Biron, 2006; Stojdl et al., 2003). Consequently, VSV harboring an M protein containing the M51R mutation is not able to efficiently spread in normal tissue whereas cancer cells, often deficient in their ability to mount an effective antiviral response due to deficiencies in the IFN pathway, are readily infected (Balachandran and Barber, 2000, 2004; Stojdl et al., 2000a). This confers VSV its exquisite oncolytic properties (Balachandran and Barber, 2000, 2004; Desforges et al., 2001- Lichty et al., 2004; Stark et al., 1998).

In the last decade, multiple viruses have been tested as oncolytic agents with some of these already in clinical trials. One conclusion emerging from these studies is the need for developing a variety of different oncolytic agents if the successfiil treatment of a wide spectrum of cancer types is to be achieved. Moreover, the development of agents with diverse oncolytic characteristics could contribute to increase our knowledge of the mechanisms involved in virus-mediated tumor regression as well as providing new tools for the treatment of specific tumor types.

In light of this, we characterized the cytopathic profiles of four VSV G mutants. Gs, G6, Gsr and Gór were previously shown to possess a wild-type M protein (Desforges et al., 2001) while still being able to inhibit cellular protein translation (Francoeur et al., 1987) without persisting in infected cells (Desforges et al., 2002). These properties render them attractive candidates for oncolytic virotherapy studies. We therefore investigated the cytopathic properties of these G mutants by analyzing their capacity to inhibit host



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