3513659534

3513659534



82

CD36, either isolated from the bonę marrow as MSC and from the bonę fragments as osteoblasts. Nevertheless, bonę cells from WT and Cd36KO mice showed comparable ALP activities suggesting that celi preparations from WT and Cd36KO mice were similar in terms of the celi populations, which was confirmed by phenotypic analysis. The involvement of CD36 in celi proliferation has been reported previously. CD36 deficiency has been shown to reduce the proliferation of astrocytes and delayed closure of the wound gap (Bao et al., 2012). Moreover, ribozyme-mediated down regulation of CD36 was shown to inhibit growth of TSPl-expressing cells (Yamazaki et al, 2004). Interestingly, our results indicate that celi survival under osteogenic culture conditions was reduced in cells deficient for CD36. Also, as celi apoptosis was not investigated, we cannot exclude that cells from Cd36KO mice are morę sensitive to apoptosis. Such impaired osteoblastic bonę formation caused by decreased number and activity of individual osteoblastic cells was shown to be responsible of reduction of bonę mass in małe patients with idiopathic osteoporosis (Ruiz-Gaspa et al., 2010).

To further investigate the functions of cells lacking CD36, we determined the expression levels of key genes of osteoblast functions (Eriksen, 2010; Lian et al., 2006). Our results indicated that bonę marrow-derived MSC from Cd36KO mice have Iow gene expression of Runx2, Osx, Ocn and Bsp when compared to WT mice whereas Col-IaJ gene expression was similar. Such impairment in osteoblastic gene expression further suggests that bonę cells functions are altered in Cd36KO mice. Given that we evidenced altered celi survival of CD36-deficient cells, reduced Von Kossa and Alizarin red stainings that was observed (data not shown) likely arise from lower density in culture dishes of cells from Cd36KO mice. Therefore, further experiments are needed to reveal mechanistic dysfunctions in CD36-deficient cells and such functional impairment are currently under investigation.



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