3513659767

3513659767



68

animals were provided with a standard mouse chow diet and drinking water. Cd36 genotyping was done by PCR as described previously (Luangrath et al., 2008) using specific    primers    for the    targeted    allele    (5’-

CAGCTCATACATTGCTGTTTATGCATG    and    3’-

CGCTTCCTCGTGCTTTACGGTATC). This study was conducted according to protocols approved by the Animal Care and Use Committee of Universite du Qućbec & Montreal.

2.5.2 Plasma analysis

Blood was collected into heparin collection tubes (68 USP, BD Bioscience) by cardiac puncture of anaesthetised mice, prior to their euthanasia. Blood was centrifuged for 30 min at 2000g and 4°C, and plasma was recovered and stored at -80°C until analysis. Plasma levels of glucose, calcium, phosphate and ALP activity were determined using QuantiChrom Assay Kits (BioAssay System, Hayward, CA, USA). Plasma concentrations of total cholesterol, high density lipoprotein (HDL)-and LDL-cholesterol were measured using EnzyChrom AF HDL and LDIWLDL Assay Kit (BioAssay System) according to the manufacturer’s instructions. Plasma levels of tartrate-resistant acid phosphatase (TRAP) isoform 5b, N-terminal propeptide of type I procollagen (PINP) and C-terminal telopeptide of Col-I (CTX) were measured by EIA assays (IDS Inc, Fountain Hills, AZ, USA). OCN detection in plasma was done using a mouse EIA kit (Biomedical Technologies Inc, Stoughton, MA, USA) according to the manufacturer’s instructions.

2.5.3 Documentation of bonę architecture

MicroCT analyses were performed using a Skyscan 1172c X-ray computed microtomograph (Skyscan, Kontich, Belgium) equipped with an X-ray tubę working



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