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'gure 4. Influence of NK celłs on CVF therapy. A, C57B1/6 mice were mjected subcutaneously with BI6 cells (n=6-8), NK-depleted or not at day 4 and treated or not with CVF at day 6. Tumor growth was measured at indicated time points. Data show the mean ± SEM and are representative of two independent experiments. *P < 0.05, **P < 0.01. B, C57B1/6 mice were injected subcutaneously with B16gp33 cells, NK-depleted or not at day 4 and treated or not with CVF at day 6. Ninę days following CVF treatment, spleen was harvested and an ex vivo stimulation with the gp33 (KAVYNFATC) peptide was performed to analyze cytokine secretion and C, degranulation. Data are the mean ± SEM of two independent experiments (n=6). D, Proposed model for the influence of complement proteins and NK cells in the B16 melanoma model. Inhibition of the complement system by CVF allows for increased NK celi availability in tumor tissue. This expanded proportion of NK cells leads to increased tumor celi lysis and antigen release thus facilitating activation of
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