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ETHYLENE GLYCOL MONOBUTYL ETHER

Figurę 3 TLC mapsof ³²P-labeIled DN A-adducts obtained from contro! and 2-butoxyethanol-treated rats. A, C, E, G and / correspond to DNA samples from control animals and B, D, F, H and J corre-spond to DNA samples from treated animals DNA. Samples were from brain (A.B), kidney (C,D), liver (E,F), spleen (G,H) and testis (I.J). First dimension was run on a separate piąte: d2, d3 and d4 correspond to dimension 2, 3 and 4 respectively.

To summarize, 2-butoxyethanol treatments of rats and transgenic mice carrying ras oncogenes did not cause any particular effects at the DNA level considering the fol-lowing endpoints: adduct level, methylation status and tumor formation at 120 days in transgenic v-Ha-ras genes. Therefore the hematological, testicular and other cellular effects which have been described previously by others (Hardin, 1983; Grant, 1985; Spano, 1991) may be due to other factors such as non-specific cytotoxicity, celi pro-liferation or apoptosis deregulation.

Acknowledgements

This work was supported by a grant (Convention N° 800384) from INRS. The skillful technical as-sistances of G. MOREL and L. DELSAUT and the pathological studies of D. ZISSU are gratefully acknowledged.

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