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P.-J. Royer et al.

408 Cul turę Medium and DC Maturation

uncicr GMP conditions, thc culnirc protocol can bc casily standardizcd. Bcsidcs, largc amount of IL-12p70 \vcrc produccd with HSA-supp!cmcntcd RPMI. Howcvcr, wc notcd that thc Thl activation was indnccd whatcvcr thc admrc medium uscd, bccaasc of poły I:C, a strong Thl induccr [31, 32]. Finally, DC functional propcrtics wcrc asscsscd by T-ccll stimulatory capacitics. Consistcnt with phcnotypic analysis, l-ccii prolifcration was highcr with 1-CS, AP and HSA than with HS-supplcmentcd RPMI. Thus, DC gcncratcd in HSA medium arc endowed with complctc functional propcrtics.

In condusion, our study providcs ncw insighcs into DC standard production for clinical usc. It highlighcs thc medium influence on DC generation and maturation and shows thc nocd to cvaluatc and validatc thc medium but also thc protein constitucnt. According to our rcsults, DC generation in a tested HSA batch-supplcmcntcd RPMI offers thc best compromisc bctwccn maturation cfficicncy and mccting safety rcquircmcnts for operating proccdurcs in clinical applications.

Acknowledgments

This work was supported by grants from INSERM, La Liguc Rcgionalc Contre Ic Canccr (Vcndćc, Morbihan, Pays dc Loirc), and Association pour la Rcchcrchc (Contre Ic Canccr. P.-J. Royer is thc rccipicnt of a fcllow-ship from La Liguc Dćpartcmcntalc Contre Ic Canccr (Loirc Atlantiquc Committcc).

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