Prace oryginalne

Mikol. Lek. 2001, 8 (1): 7-12

ISSN 1232-986X

Circulating antibodies against Trichophyton rubrum antigens

in sera of patients with tinea

Kr¹¿¹ce przeciwcia³a przeciw antygenom Trichophyton rubrum u chorych na grzybice skóry

Eugeniusz Baran, Anita Hryncewicz-GwóŸdŸ, Rafa³ Bia³ynicki-Birula, Ewa Plomer-Niezgoda, Maria Cis³o,

Katarzyna Pó³grabia-Szwedo

Katedra i Klinika Dermatologii i Wenerologii AM we Wroc³awiu

It was assumed that exposition to the mycotic antigen in the course of the mycotic infection leads to the humoral response.

The purpose of the study was to examine whether circulating IgG and IgM antibodies against T. rubrum antigens detected by immunoblotting

(SDS-PAGE) are present in the sera of patients with skin or nail mycosis. Another aim of our project was to compare the occurrence of various types of

antibodies IgG and IgM against mycotic antigens in patients with mycosis with the antibody profile of the healthy subjects (control group) and of the

patients with atopic dermatitis (AD).

Material and methods: 35 patients with skin or nail fungal infection were examined. Mycosis was confirmed in direct examination and by culture.

T. mentagrophytes was found in 11, T. rubrum in 6, M. canis in 3, C. albicans in 10, Scopulariopsis brevicaulis in 2 cases. Geotrichum candidum

was present in 1 patient. The cultures of 2 patients were negative. The control group consisted of 25 healthy subjects and 19 patients with atopic der-

matitis. Immunoblotting was applied as a very sensitive method allowing to determine antibodies against various antigens of the ethanol extract of

T. rubrum cultures (exoantigens). Fischer’s test was used for statistical evaluation of the study results.

Results: Antibodies against various T. rubrum antigens were found in the examined sera. The most frequent antibodies were those against antigens of

molecular weight 29.5 kD and 25.7 kD, and against two groups of antigens of molecular weight about 17.8 kD and about 8.9 kD. Antibodies against

25.7 kD antigen were present in the majority of examined subjects: patients with mycosis (31 out of 35), patients with atopic dermatitis (18 out of

19), and healthy subjects (24 out of 25). Antibodies against 29.5 kD antigen were present in 20 of 34 examined patients with mycosis, only in 2 sub-

jects in the control group and in 4 with atopic dermatitis. The frequency of these antibodies appearance was statistically higher in patients with myco-

sis than in the healthy subjects and in patients with atopic dermatitis. Antibodies against 8.9 kD antigen group were present more frequently in

patients with mycosis than in the control group or patients with atopic dermatitis.

Conclusions: 1. Presence of antibodies against antigens of molecular weight 25.7 kD in majority of examined subjects, both suffering from mycosis and

healthy ones, may be a proof of a wide spread of fungi in the environment, or cross-reactivity with common antigens. 2. Antibodies against antigens

with molecular weight 29.5 kD seem to be specific in cases of mycoses (p<0.05). These mycotic antigens induce cross-reactions against various

fungi.

Key words: Trichophyton rubrum, immunoblotting, IgG, IgM

Za³o¿ono, ¿e w wyniku zaka¿enia Trichophyton rubrum dochodzi do powstania swoistej odpowiedzi humoralnej.

Cel pracy: Zbadanie, czy wystêpuj¹ swoiste kr¹¿¹ce przeciwcia³a IgG i IgM skierowane przeciw antygenom T. rubrum u chorych na grzybice skóry

i/lub paznokci.

Materia³ i metody: Poniewa¿ u chorych na atopowe zapalenie skóry (AZS) czêsto wystêpuj¹ infekcje, m.in. grzybicze, które mog¹ przebiegaæ bezobja-

wowo lub sk¹poobjawowo, utworzono dwie grupy kontrolne. W sk³ad pierwszej wchodzi³y osoby zdrowe (25 badanych), a drugiej – chorzy na AZS

(19 pacjentów). Przebadano 35 pacjentów z grzybic¹ skóry i/lub paznokci stóp. Grzybice potwierdzono bezpoœrednim badaniem mikologicznym oraz

hodowl¹. Uzyskano nastêpuj¹ce hodowle: T. mentagrophytes – 11 przypadków, T. rubrum – 6, M. canis – 3, C. albicans – 10, Scopulariopsis brevi-

caulis – 2, Geotrichum candidum – 1. U 2 chorych wynik hodowli by³ ujemny, ale wobec jawnych objawów klinicznych i stwierdzenia grzybni w bada-

niu bezpoœrednim w³¹czono te osoby do grupy badanej. W badaniach u¿yto bardzo czu³ej i swoistej metody immunoblotingu (SDS-PAGE), umo¿liwiaj¹-

cej wykrycie kr¹¿¹cych przeciwcia³ w niskich mianach. Jako antygenu u¿yto ekstraktu glikolowego z substancji uwalnianych z hodowli p³ynnej T. ru-

brum na pod³o¿u Sabourauda. Do obliczeñ statystycznych u¿yto dwustronnego testu Fischera.

7

Abstract

Streszczenie

8

Eugeniusz Baran et al.

Mikol. Lek. 2001, 8 (1)

Introduction

Infections caused by dermatophytes are limited to the

keratin layer of the epidermis, hair and nails. Both in the

acute and chronic infections immunological response

develops. Specific and unspecific reactivity reactions are

present. Specific reactivity to dermatophyte antigens has

been the subject of multiple in vitro and in vivo studies so

far. Cellular response was shown to play an outstanding

role to control dermatophyte infection (1), but in the sera

of infected patients antibodies against the fungal antigens

were also demonstrated. Circulating antibodies were

determined by various methods, and therefore the results

of the studies are equivocal. Frequency of specific anti-

bodies in the sera of patients with dermatophyte infection

was shown to be from 10% to even 100% (2-7). Derma-

tophytes are characterised by the complex structure of

mycelium and the secreted substances. The antibody

composition of one dermatophyte strain may differ de-

pending on the duration of the culture, growth conditions

like the type of the medium or temperature (8). Modern

methods were applied to evaluate the biochemical structure

of dermatophyte extracts in the recent studies. Electro-

phoretic diffusion of Trichophyton mentagrophytes myce-

lium extract showed 25 bands that corresponded with the

proteins of molecular weight from 98 to 12 kD. 17 of those

25 proteins were found to stimulate the immunological

response in immunized animals (9).

The aim of our study was to evaluate the occurrence

of the antibodies against individual elements of the anti-

gens extracted by T. rubrum. Those antigens were at first

divided by the electrophoretic diffusion on the polyacry-

lamid gel (SDS-PAGE). Another aim of the project was to

examine whether the population of patients with mycoses

differs from the population of healthy subjects and the

group of patients with the atopic dermatitis (AD) as far as

the production of those antibodies is concerned. Patients

with AD were included in the study as this group of pa-

tients is very susceptible to various infections, also the

fungal ones. The humoral response to dermatophyte anti-

gens in this group of patients seemed extremely interesting.

Materials and methods

35 patients with skin and/or nail mycosis, aged 17-

-84 (mean age 45.2) were studied. 13 patients were

females (mean age 38.4), 22 – males (mean age 51.4).

19 patients with atopic dermatitis aged 17-52 (mean age

31.9) were also studied. Mycosis was confirmed by the

direct examination and by the culture. Following cultures

were obtained: T. mentagrophytes (11 cases), T. rubrum

Wyniki: W badanych surowicach stwierdzono wystêpowanie przeciwcia³ przeciw wielu antygenom T. rubrum. Ustalono, ¿e najczêœciej wystêpuj¹ce prze-

ciwcia³a s¹ skierowane przeciwko antygenom o masie cz¹steczkowej 29,5 kD, 25,7 kD oraz grupie antygenów o masach bliskich 17,8 kD i 8,9 kD. Wyka-

zano, ¿e przeciwcia³a przeciw antygenowi o masie 25,7 kD wystêpuj¹ u wiêkszoœci badanych, zarówno u chorych na grzybice (31 spoœród 35 badanych),

chorych na AZS (18 spoœród 19 badanych), jak i u osób zdrowych (24 spoœród 25 badanych). Przeciwcia³a przeciwko antygenowi o masie 29,5 kD

stwierdzono u 20 na 34 badanych pacjentów z grzybic¹ oraz jedynie u 2 osób z grupy kontrolnej i 4 chorych na AZS. Wykazano ró¿nicê statystycznie

istotn¹ (p<0,05) miêdzy wystêpowaniem tych przeciwcia³ u pacjentów z grzybic¹ a grup¹ kontroln¹ i chorych na AZS. U chorych z grzybic¹ równie¿ czê-

œciej ni¿ w grupie kontrolnej i u chorych na AZS wystêpowa³y przeciwcia³a przeciw grupie antygenów o masie 8,9 kD.

Wnioski: Wystêpowanie przeciwcia³ przeciw antygenowi o masie 25,7 kD u wiêkszoœci badanych osób, zarówno cierpi¹cych na grzybice, jak i wolnych od

tej infekcji, mo¿e œwiadczyæ o szerokim rozpowszechnieniu grzybów w œrodowisku lub o krzy¿owej reaktywnoœci z powszechnie wystêpuj¹cymi antygena-

mi. Przeciwcia³a przeciw antygenowi o masie 29,5 kD wydaj¹ siê specyficzne dla infekcji grzybiczych (p<0,05), a ich oznaczanie mo¿e staæ siê praktycz-

nym testem serologicznym potwierdzaj¹cym infekcje grzybicze. Antygen ten ma w³aœciwoœci wywo³ywania reakcji krzy¿owej u chorych z grzybic¹ spowo-

dowan¹ przez ró¿ne rodzaje grzybów.

S³owa kluczowe: Trichophyton rubrum, immunobloting, IgG, IgM

Antibodies against specific antigens of T. rubrum extract

Przeciwcia³a przeciw specyficznym antygenom ekstraktu T. rubrum

n

29.5 kD

25.7 kD

17.8 kD

8.9 kD

antigen group

antigen

grupa

group

antygenu

grupa

antygenu

Patients with

35

20

31

5

16

mycoses

(57.1%)

(88.6%)

(14.2%)

(45.7%)

(total)

Chorzy na

grzybicê

(ogó³em)

Patients with

mycosis

caused by:

Chorzy na

grzybicê

wywo³an¹

przez:

T. mgr

11

5

11

1

6

T. rubrum

6

4

5

2

2

M. canis

3

2

3

1

2

C. albicans

10

5

10

0

2

Scopulariopsis

2

1

1

1

1

Geotrichum

1

1

0

0

1

Negative

2

2

1

0

2

culture

Hodowle

ujemne

AD

19

4

18

0

6

AZS

(21%)

(94.7%)

(31%)

Control group

25

2

24

8

9

Grupa

(8%)

(96%)

(32%)

(36%)

kontrolna

Table I: The occurrence of antibodies against specific antigens of T. rubrum extract (29.5

kD, 25.7 kD, 17.8 kD, 8.9 kD) in the sera of patients with mycoses, atopic dermatitis

and in healthy control group

Tabela I: Wystêpowanie przeciwcia³ przeciw specyficznym antygenom ekstraktu T. rubrum

(29,5 kD, 25,7 kD, 17,8 kD, 8,9 kD) w surowicy chorych na grzybice, atopowe

zapalenie skóry i u osób zdrowych

9

Circulating antibodies against Trichophyton rubrum antigens in sera of patients with tinea

(6 cases), M. canis (3 cases), C. albicans (10 cases),

Scopulariopsis brevicaulis (2 cases), Geotrichum can-

didum (1 case). In 2 patients the culture turned out to be

negative. The duration of mycotic infection varied

from 3 weeks to 35 years. Mycotic infection was found in

3 patients with atopic dermatitis and these patients were

included in the group of patients with tinea and exluded

from AD group.

The control group consisted of 25 healthy subjects

aged 21-52 (mean age 33). None of the subjects decla-

red any symptoms of mycosis in anamnesis as well as

any features of dermatomycoses were revealed in physi-

cal examination.

The preparation of the extract of T. rubrum was done

according to Garg et al. method (9). The supernatant of

the 4-week culture on the liquid Sabouraud medium was

filtered trough the Whatmann 3 filter paper. Filtrate was

20 times condensed by the method of ultrafiltration, then

rinsed with 2 volumes of distilled water and enriched with

95% ethanol to achieve the 80% concentration solution.

The precipitate was kept for 42 hours in the temperature

of 4°C and mixed incessantly, then centrifuged at 6000

rotations per min. for 30 minutes. The sediment was dis-

solved in 60 ml demineralised distilled water, centrifuged

at 2000 rotations per min. in order to obtain the insoluble

particles, which were finally lyophylized. Antibodies were

determined by immunoblotting according to Laemmli’s

method (10). 12% separating gel and 4% condensing gel

(gel composision: acrylamid/bisacrylamid 30:0.8) were

used for elecrophoresis. The antigen was reduced in

100°C for 4 min. under 5 times concentrated experimen-

tal buffer. 40 µg of antigen was added to each well.

Electrophoresis duration was 1 hour, the voltage 200 V,

and buffer of pH 8.3 was applied. The separated antigen

was transferred to nitrocellulose and incubated with the

examined sera after their dilution 1:1000. The examined

antibodies were demonstrated by means of the rabbit

antibodies against the human IgG and IgM coniugated

with alkaline phosphatase (DAKO) in the concentration

1:2000. Substrates NCT and BCIP (SIGMA) were used

for colour reaction. Fischer’s test was used for statistical

evaluation of the study results.

Results

The occurrence of antibodies against several antigens

of T. rubrum was shown in the studied sera. Antibodies

against antigens of the molecular weight of 29.5 kD and

25.7 kD and against the antigen group of molecular

weight of 17.8 kD and 8.9 kD were found to be most fre-

quent. It was demonstrated that antibodies against anti-

gen of 25.7 kD molecular weight were present in most of

the examined subjects with mycosis (88.6%), atopic der-

matitis (94.7%) and in the healthy control group (96%)

(tab. I, fig. 1). Antibodies against antigen of 29.5 kD mole-

cular weight were present in 20 of 34 studied patients

(57.1%) with mycosis, and only in 2 (8%) healthy subjects

and in 4 (21%) with atopic dermatitis. The difference in

the occurrence of these antibodies was statistically signifi-

cant (p<0.05) when patients with mycosis were compa-

red to the control group and to the patients with atopic

dermatitis (tab. I, fig. 1-4). When the type of the mycotic

infection was taken into consideration patients were divided

into those infected by dermatophytes, yeasts and others.

No statistical difference in the occurrence of antibodies

against particular T. rubrum antigens was found (tab. II).

As the next step the analysis of simultaneous occurrence

of antibodies against antigens of 29.5 and 25.7 kD in the

Antibodies against specific antigens of T. rubrum extract

Przeciwcia³a przeciw specyficznym antygenom ekstraktu T. rubrum

n

29.5 kD

25.7 kD

17.8 kD

8.9 kD

antigen

antigen

group

group

grupa

grupa

antygenu

antygenu

Patients

35

20

31

5

16

with mycoses

Chorzy

na grzybicê

Patients with

20

11

19

4

10

dermatophyte

caused

infection

Chorzy

na zaka¿enie

wywo³ane

przez

dermatofity

Patients with

10

5

10

0

2

yeast infection

Chorzy

na zaka¿enia

wywo³ane

przez

dro¿d¿aki

Other

5

4

2

1

4

Inne

przypadki

Table II: The occurrence of antibodies against specific antigens of T. rubrum extract

(29.5 kD, 25.7 kD, 17.8 kD, 8.9 kD) in the sera of patients with mycoses caused

by dermatophytes, yeasts and other fungi

Tabela II: Wystêpowanie przeciwcia³ przeciw specyficznym antygenom ekstraktu T. rubrum

(29,5 kD, 25,7 kD, 17,8 kD, 8,9 kD) w surowicy chorych na grzybicê wywo³an¹

przez dermatofity, dro¿d¿aki i inne grzyby

Antibodies against specific antigens of T. rubrum extract

Przeciwcia³a przeciw specyficznym antygenom ekstraktu

T. rubrum

29.5 kD

%

only

%

only

%

none

%

+25,7 kD

29.5 kD

25.7 kD

brak

Patients with

16

45.7%

4

11.4%

15

42.9%

0

0%

mycoses

Chorzy

na grzybicê

AD / AZS

4

21%

0

0%

14

73.7%

1

5,2%

Control group

2

8%

0

0%

22

88%

1

4%

Grupa kontrolna

Table III: The occurrence of antibodies against specific antigens of T. rubrum extract of

29.5 kD and 25.7 kD in the sera of patients with mycoses, atopic dermatitis and in

healthy control group

Tabela III: Wystêpowanie przeciwcia³ przeciw specyficznym antygenom ekstraktu T. rubrum

(29,5 kD and 25,7 kD) w surowicy chorych na grzybice, atopowe zapalenie skóry

i u osób zdrowych

10

Eugeniusz Baran et al.

Mikol. Lek. 2001, 8 (1)

examined sera was performed. Their simultaneous occur-

rence was recorded in 16 (45.7%) patients with mycosis

and in only 2 (8%) subjects from the control group (the

difference of statistical importance by p<0.05) and in 4

cases with atopic dermatitis (21%). Antibodies against

antigen of 29.5 kD with no accompanying presence of

antibodies against 25.7 kD antigen were found in 4

(11.4%) patients with mycosis. This was not observed

either in the healthy control group or in patients with

atopic dermatitis (tab. III, fig. 2-4).

In patients with mycosis antibodies against antigen

25.7 kD are often accompanied by antibodies against

antigen 29.5 kD. In healthy population and in patients with

atopic dermatitis antibodies against antigen 25.7 kD are

only rarely accompanied by antibodies against 29,5 kD.

Antibodies against antigens of 8.9 kD molecular weight

were more frequently observed in patients with mycosis

than in the control group and in patients with atopic der-

matitis (tab. I). This difference, however, was not statisti-

cally significant. There was also no statistically important

difference in the occurrence of antibodies against anti-

gens of 17.8 kD between the 3 studied groups.

Discussion

The results of our study show that antibodies against

antigens of T. rubrum occur in the sera of patients with

mycosis as well as in the sera of subjects with no clinical

symptoms and no history of the fungal infection. The

studies of other authors have been equivocal up to the

present as far as the specific antibodies against dermato-

phytes in humans are concerned. This divergence of the

records might result from the sensitivity of the method

used to determine the antibodies, like double immunodif-

fusion (11, 12), immunoelectrophoresis (13), comple-

ment binding test (14), precipitation (2), immunofluores-

cence (15), and ELISA (3, 7, 16). The method applied

for antigen extraction might also be important (8). The

application of a very sensitive method as immunoblotting,

allowed to discover even very low concentrations of IgG

and/or IgM antibodies specific for T. rubrum in the studied

sera. The occurrence of these antibodies was found in all

studied groups, both in patients with mycosis and the

subjects with no fungal infection. Similar results were

described by Honbo et al. (7) who used ELISA test for

the study. There might be several reasons of such com-

mon presence of fungal antibodies in the population.

Earlier exposition to dermatophyte antigens might stimu-

late antibody production or cross-reaction with the anti-

gens of other fungi (yeasts, moulds) (11) or bacterial anti-

gens (17), and even with the human antigens like inter-

cellular glycoprotein or erythrocyte isoantigen A (18). The

contrary results were shown by Hamouda et al. (3) and

Kaaman et al. (16) who described increased concentra-

Fig. 1. The occurrence of antibodies against

T. rubrum antigens in the studied sera

Ryc. 1. Wystêpowanie przeciwcia³ przeciw an-

tygenom T. rubrum w badanych surowi-

cach

Sera of patients with mycoses / Surowice chorych na grzybicê
Sera of patients withatopic dermatitis / Surowice chorych na atopowe zapalenie skóry
Sera of control group / Surowice grupy kontrolnej

100

90
80
70
60
50
40
30
20
10

0

29.5

Antibodies against studied antigens [M.W. of antigens in kD]

Przeciwcia³a przeciw badanym antygenom [ciê¿ar cz¹steczkowy antygenów w kD]

Frequence of antibodies occurrence [%] Czêstoœæ wystêpowania przeciwcia³

25.7

17.8

8.9

Fig. 2. Antibodies against antigens of molecular weight of 29.5 kD and 25.7 kD

in sera of patients with mycoses

Ryc. 2. Przeciwcia³a przeciw antygenom o ciê¿arze cz¹steczkowym 29,5 kD

i 25,7 kD w surowicy chorych na grzybice

Ab only against 25.7 ag

Przeciwcia³a tylko prze-

ciw antygenom 25,7 ag

Ab only

against 29.5 ag

Przeciwcia³a

tylko przeciw

antygenom 29,5 ag

Ab against 29.5 kD +

+ 25.7 kD ag

Przeciwcia³a przeciw

antygenom 29.5 kD +

+ 25.7 kD ag

42.9%

11.4%

45.7%

11

Circulating antibodies against Trichophyton rubrum antigens in sera of patients with tinea

tion of specific IgG in patients with dermatophytosis when

compared to healthy subjects. Our analysis of antibodies

against individual components of antigens secreted by

T. rubrum gave interesting results. The analysis was

based on the results of electrophoretic diffusion. In the

studied sera antibodies against antigens of molecular

weight from 8 kD to approximately 29.5 kD were deter-

mined. Antibodies against antigens of 29.5 kD and 25.7

kD were the most characteristic. All other determined

antibodies were categorised into one of the two groups,

as the interpretation of multiple individual strains was

extremely difficult. Antibodies against antigen of 29.5 kD

were found in 20 (57.1%) patients with mycosis, in 2

(8%) healthy subjects and in 4 (21%) patients with atopic

dermatitis, whereas antibodies against antigen of 25.7 kD

were present in the majority of the examined subjects

except for 1 (4%) healthy subject, 4 (11.4%) patients with

dermatophytosis and 1 (5.3%) patient with AD. The fre-

quency of occurrence of antibodies against antigen 29.5

kD was statistically significantly higher in patients with

dermatophytosis than in the control group. Determination

of this antibody is more specific than the determination of

other antibodies, and thus it could be applied in the clinical

diagnosis. Patients with atopic dermatitis are known to be

more susceptible to various infections, and the fungal ones

as well. The sera of patients with atopic dermatitis were

therefore examined for the occurrence of antibodies against

dermatophytes. However, the clinical symptoms of der-

matophyte infection confirmed by the mycological examina-

tion were found only in 3 cases, antibodies against antigen

29.5 were present in a greater percentage of the patients

than in the control group, although the patients did not have

history of mycological infection. The reason of this observa-

tion is not clear at the moment. Perhaps the skin of these

patients is more often colonised by this group of fungi or

patients are characterised by higher reactivity to shorter

exposition to the antigens.

Further analysis of the results made us divide all stu-

died subjects into 3 groups: with dermatophyte infection,

with yeasts infection and other. No statistically significant

difference was found between those groups as far as the

occurrence of antibodies against individual antigens of

T. rubrum is concerned (tab. II), which might be the result of

cross-reaction between those antigens. Other authors simi-

larly to us described the cross-reactions between antigens of

various species of dermatophytes (11, 19).

Piœmiennictwo

1. Jones H.E., Reinhardt J.H.: Model dermatophitosis in naturally

infected subjects. Arch. Dermatol., 1974, 110, 369-374.

2. Svejgaard E., Christansen A.H.: Precipitating antibodies in der-

matophytosis demonstrated by crossed immunoelectrophoresis.

Acta Path. Microbiol. Scand. Sect. C, 1979, 87, 23-27.

3. Hamouda T., Jeffries C.D., Ekladios E.M., El-Mishad A.M., El-

Koomy M., Saleh N.: Class-specific antibody in human dermato-

phytosis reactive with Trichophyton rubrum derived antigen. My-

copathologia, 1994, 127, 83-88.

4. Woodfolk J. A., Slunt J.B., Deuell B., Hayden M.L., Platts-Mills

T.A.E.: Definition of a Trichophyton protein associated with de-

layed hypersensitivity in humans. J. Immunol., 1996, 156, 1695-

-1701.

5. Davies R.R., Ganderton M.A., Savage M.A.: Human nail dust and

precipitating antibodies to Trichophyton rubrum in chiropodists.

Clin. Allergy, 1983, 13, 309-315.

6. Leibovici V., Evron R., Axelrod O., Westerman M., Shalit M., Barak

V., Frankenburg S.: Imbalance of immune respons in patients

with chronic and widespread fungal skin infections. Clin. Exp.

Dermatol., 1995, 20, 390-394.

7. Honbo S., Jones H.E., Artis W.M.: Chronic dermatophyte infec-

tion: Evaluation of Ig class-specific antibody response reactive

with polysaccharide and peptide antigens derived from Tricho-

phyton mentagrophytes. J. Invest. Dermatol., 1984, 82, 287-

-290.

8. Haan P., Raay-Helmer E.M.H., Boorsma D.M.: Diversity of anti-

genic extracts from the dermatophyte Trichophyton rubrum. My-

kosen, 1986, 9, 427-433.

9. Garg A.P., Muler J.: Preparation of antigens from Trichophyton

mentagrophytes using a new semi-solid culture medium and

their characterization by SDS-PAGE and immunological tech-

niques. Mycoses, 1992, 35, 349-355.

10. Laemmli U.K.: Cleavage of structural proteins during the assembly

of the head of bacteriophage T4. Nature, 1970, 227, 680-685.

Fig. 3. Antibodies against antigens of molecular weight of 29.5 kD and 25.7 kD

in sera of patients with atopic dermatitis

Ryc. 3. Przeciwcia³a przeciw antygenom o ciê¿arze cz¹steczkowym 29,5 kD

i 25,7 kD w surowicy chorych na atopowe zapalenie skóry

Without antibodies

Bez przeciwcia³

Ab only against

25.7 kD ag

Przeciwcia³a tylko przeciw

antygenom 25,7 kD ag

Ab against

29.5 kD +

+ 25.7 kD ag

Przeciwcia³a

przeciw antyge-

nom 29.5 kD +

+ 25.7 kD ag

73.7%

5.3%

21.0%

Fig. 4. Antibodies against antigens of molecular weight of 29.5 kD and 25.7 kD

in control group

Ryc. 4. Przeciwcia³a przeciw antygenom o ciê¿arze cz¹steczkowym 29,5 kD

i 25,7 kD w grupie kontrolnej

Without antibodies

Bez przeciwcia³

Ab only against

25.7 kD ag

Przeciwcia³a tylko przeciw

antygenom 25,7 kD ag

Ab against

29.5 kD +

+ 25.7 kD ag

Przeciwcia³a

przeciw antyge-

nom 29.5 kD +

+ 25.7 kD ag

88%

4%

8%

Eugeniusz Baran et al.

Mikol. Lek. 2001, 8 (1)

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Address for correspondence:

Prof. dr hab. Eugeniusz Baran

Katedra i Klinika Dermatologii i Wenerologii AM

ul. T. Cha³ubiñskiego 1

50-368 Wroc³aw, Poland

Received: 08-12-2000

Approved: 9-01-2001