Typically, for the production of
human therapeutic proteins, a
stainless steel bioreactor
constructed of the best quality
(316) virgin steel with a working
volume of 2000 to 5000 liters is
used. However, stainless steel
bioreactors with working
volumes of up to 100,000 liters
are also in use today.

Tank do produkcji
penicyliny przez
Penicylium chrysogenum

CSTR  - mechanical mixing and air-sparged liquid media

Airlift - uses air sparging to pneumatically mix the media

Combinations and variations of CSTR and Airlift reactors

Membrane - two permeable membranes to deliver nutrients and

export wastes and products from bacteria in a center tube

TYPES OF BIOREACTORS

TYPES OF BIOREACTORS

CSTR  - mechanical mixing
and air-sparged liquid
media
Airlift - uses air sparging to
pneumatically mix the
media
Combinations and
variations of CSTR and
Airlift reactors
Membrane - two
permeable membranes to
deliver nutrients and
export wastes and
products from bacteria in a
center tube

pH Control Loop

Dissolved Oxygen Control Loop

Przykładowy przebieg reakcji w bioreaktorze

Glukoza

Żywe komórki

Martwe komórki

mleczan

Produkt ogółem

Vaccine production plant bioreactors

For specific stimulation the following parameters have been
investigated: kind of antigen-presenting cell, incubation time with the
antigen, cell density, ratio between APC and T cells, the influence of
serum and IL-2 and the course of restimulation. Using the optimized
protocol the portion of hCMV specific cells could be increased from 1.1%
to 23.4% during four weeks and a final number of 7.5*107 hCMV
specific T-cells has been generated