095
vector
(Single type of molecule)
(Numerous different fragmenls.,.1.2,3. etc.)
1
3
2
Recombinant DNA molecules
| DNA transformation jl Replication within celi
Each celi contains only one type of recombinant DNA
Platę out on nutrient agar
Each colony is a population of identical bacterial cells (clones) containing a specific recombinant DNA molecule
|
n |
f=? |
n |
|
W i | ||
|
A |
A |
A |
|
ł |
1 |
1 |
|
O 1 |
02 |
03 |
|
lndividual DNA clones | ||
1. Expand in culture for individual colonies
2. Purify recombinant DNA
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