4

Summarized test proceduro

iOHXX strongly recommends that you rsad the complete instructions carefully before using the test the first time.

Step	Action	■—-~-~r
1, Sample Preparatlon and Dlstributłon	-    Dilute test samples flve hundred fold (1:500) włth sample diluent prior to being asayedtal by diluting 1 pi of sample włth 500 pl of sample diluent). NOTĘ: 00 NOT DILUTE CONTROLS. Be surę to change tips for each sample. Samples must be thoroughly ndxed pdor to dlspensing into the coated piąte. -    Reagents should be allowed to come to room temperaturo, then mixed by irwerting and swirllng. -    Dispense 100 pl of undiluted negathre control into appropriate welis. -    Dispense 100 pl of undiluted positive control into appropriate wells. -    Dispense 100 pl of diluted sample into applicable wells.
2. Sample Incubation	- incubate for 30 minutes at room temperaturo.
3. Washlng the Platę	- Wash each well with approximately 350 pl of dlstilled or deionlzed water S-5times.
4. Conjugate Dlstributłon	- Dispense 100 pl of Antl-Chicken/Anti-Turkey Horseradlsh Peroxidase conjugate into each wad.
5. Conjugate Incubation	- Incubate for 30 minutes at room temperaturo.
6. Repeat Step 3
7. Substrate Dlstributłon	- Dispense 100 pl of TMB substrate solution into each well.
8. Substrate Incubation	- Incubate for 15 minutes at room temperaturo.
9. Stopping the Reactłon	- Dispense 100 pl of stop solution Into each well to stop the reactłon.
10. Measure the Plata	*    Blank the reader with air. *    Measure and record absorbance values at 650 nm, A(650).
11. Interpretation (SI?)	s 0.2	> 0.2 (tlters >396)__
1___	negatlye	posltłve (yaccination or exposure to APV)__

Manutactured by;

IDEXX Swłtzwiand AG

Stationsstrasse 12

3097 Uebefold-Bern, Swltzerland

For technlcal assistance

Contact your IDEXX area manager or dlstributor or vlsit:

www.ldexx.com/production/contact IDEXX Technlcal Support: 00-800-727*43399