Antigon is itttcM to bottom of vtll
►
Płlitofi sorum is addod. If antibodios
antbuman globuln (AHG) wrth onzymo attachod ts addod
Substrato of onzymo is addod. If onzymo is prosont, a co lor od product will bo formod. Co ter indicatos tho antibody is prosont.
to tho antigoo aro prosont, thoy will bind to antięon. Plato is washod.
Plato is again _ washod. AHG will romain onły if antbody is prosont.
O ■ antigon
Y ■ ant body A = antbuman globulin
□ ■ onzymo