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CeII Transport Lab Series

GLUE IN THE FOLLOWING PROCEDURES AND CREATE DATA TABLES IN YOUR LAB MANUAŁ! COMPLETE THE EXPERIMENT. THEN ANSWER THE ANALYSIS QUESTIONS FOR EACH SECTION.

I. Gummy Bcars

1.    Obtain 3 gummy bcars and threc cups. Label them sugar, watcr, nonę and writc your names/initials on thc cups.

2.    Crcatc a data tablc. Find the mass of each bcar. Rccord the generał appearance of each.

3.    Place one in 20mL syrup, the other in 20mL salt water, and one in no water. Leave overnight.

4.    Biot the bears dry. Record the mass and the generał appearance again.

Lab Analysis:

1.    Create a graphic organizer to compare and contrast the changes in the “gummies" using the terms hypertonic, hypotonic and isotonic to describe changes observed.

2.    Identify the Independent Variable_ Dependent Variable_

Control Group_Constants:_

U, Diffusion across a selectively permeable membranę

1. Create a data table in your lab book:

	Initial color	Color after 25 minutes
Inside the dialysis tubing
Liguid in the beaker

2.    Open the tubing. (ftub the WET tubing in between your fingers in order to get an opening at one end.)

3.    Twist the other end of the tubing and tie it closed with a string.

4.    Sguirt two pipettes fuli of starch solution into the tubing. Be careful not to let any starch solution come in

contact with the outside of the tubing bag. Record the color of the starch in a data table.

5.    Twist and tie the other end closed. with string.

6.    Rinse the outside of the tubing.

7.    Record initial colors in the data table.

8.    Place the tubing into a beaker half-f illed with water and then add about 15 drops of iodine. Record the color of

the water/iodine solution in your data table.

9.    Af ter about 25 minutes. record the colors of the iodine/water solution in the beaker and the color of the

starch inside the bag.

10. CIean Up. Throw the dialysis tubing in the garbage. Rinse out beakers and put away.

Lab Analysis:

1.    Describe the color change (if any) that occurred within the dialysis tubing between the start and at the end of the experiment.

2.    What was the color of the iodine outside the dialysis tubing at the start and at the end of the experiment?

3.    What part of a celi does the dialysis tubing represent?

4.    What color is formed when iodine and starch mix together?

5.    Did iodine move into the bag? Provide evidence based on your results.

6.    Did starch move out of bag? Provide evidence based on your results.

7.    What does permeable mean? Is the dialysis tubing permeable to iodine? Permeable to starch? Explain how you know.

8.    Did iodine move by diffusion? Explain your answer.

9.    Porę size may determine why some Chemicals can or cannot pass through a celi membranę. How might the size of the membranę porę compare to the size of the iodine molecules? The starch molecules?

10.    How does this simulation relate to the structure and f unction of the plasma membranę? Describe what substances can move freely into and out of the membranę. Does size determine if they can move in or out? What else might be a factor?